Serum, Plasma, Cell culture supernatant
50 µl/well for all four analytes in the panel
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date.
- IFNγ : 8000 - 10.97 pg/ml
- IL-1β : 14000 - 19.20 pg/ml
- IL-6 : 5000 - 6.86 pg/ml
- TNFα : 3000 - 4.12 pg/ml
Actual values may vary from kit to kit. Please see the antigen card included in your kit for specific values.
Limit of Detection
- IFNγ : 5.40 pg/ml
- IL-1β : 12.41 pg/ml
- IL-6 : 2.90 pg/ml
- TNFα : 3.40 pg/ml
- For research use only
- Detection Method: Chemiluminescent
- Analytes: IFNγ, IL-1β, IL-6, TNFα
- This kit is validated for use with plasma, serum, and cell culture supernates
Cardiovascular disease, Coronary artery disease, Cytokines and chemokines and related molecules, Diabetology - Other Relevant Products, Immune Response, Infection and Inflammation, Neural tissue markers, Oncology, Transplantation, Energy metabolism and body weight regulation, Sepsis
The multiplexed ELISA kits, called Q-Plex™ measure the concentration of multiple proteins in each sample. Quantify cytokines, chemokines, hormones, and other common disease indicating proteins with standard or custom kits. Q-Plex Arrays are built by printing nano spots in defined arrays of multiple distinct capture antibodies in each well of a multiwell plate. Multiplexed ELISAs maximize data generation from each sample, and provide a low cost per data point.
Traditional Enzyme-Linked Immunosorbent assays (ELISA), have been used for decades in research and development, disease diagnosis and other critical fields in science. Multiplexed ELISAs offer the same time proven reliability with updated efficiency. Mutliplexed ELISAs have been developed to provide the sensitivity of ELISA with the ability to test for multiple analytes at the same time. With Q-Plex scientists can measure up to 25 distinct analytes in each well of a 96-well plate.
The Q-Plex Mouse Cytokine Panel 2 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IFNγ, IL-1β, IL-6, TNFα).
Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Q-Plex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Using just 50 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 2.5 hours. Q-Plex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.
Safety Information (RUO)