Total 335 AA. MW: 37.5 kDa (calculated). UniProtKB acc.no. Q5VYX0 (Ala18-Ile342). N-terminal His-tag (10 extra AA). Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
Purity as determined by densitometric image analysis: > 95%
< 1.0 EU/µg
Filtered (0.4 μm) and frozen from 0.3-0.5 mg/ml solution in 15 mM Tris, 85 mM glycine, 20%(w/v) glycerol, pH 8.5
Defrost at ambient temperature
Western blotting, ELISA
On ice. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for three days.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
This product is intended for research use only.