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Resistin Rat ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, RETN, FIZZ3, HXCP1, RSTN, UNQ407/PRO1199
  • Species:Rat
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Cat. No. Size Price


RD391016200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum

Sample Requirements

5 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.25–20 ng/ml

Limit of Detection

Analytical Limit of Detection is calculated from the real Resistin values in wells and is 0.05ng/ml

Intra-assay (Within-Run)

n = 8; CV = 5.1%

Inter-assay (Run-to-Run)

n = 5; CV = 7.1%

Spiking Recovery

92,10%

Dilution Linearity

112,70%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • goat Yes
  • monkey Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • human Non-detectable
  • chicken Not tested
Summary

Features

  • It is intended for research use only
  • The total assay time is less than 4 hours
  • The kit measures total serum (homodimeric) resistin
  • Assay format is 96 wells
  • Quality Controls are recombinant rat resistin based
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Animal studies

Summary

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of
cysteine-rich secreted proteins which is termed the RELM family, and is also described as
ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone).
Rat resistin contains 114 amino acids as a prepeptide, and its hydrofobic signal peptide is
cleaved before its secretion. Resistin circulates in rat blood as a dimeric protein consisting of
two 96 amino acid polypeptides, which are disulfide-linked.
Resistin may be an important link between obesity and insulin resistance. Mouse resistin,
specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes,
hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan
et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake.
They have also suggested that resistin is present at elevated levels in blood of obese mice,
and is down regulated by fasting and antidiabetic drugs (TZDs). On the other hand, several
studies demonstrated reduced resistin expression in adipose tissue of obese mice and
increased levels in leptin deficient ob/ob mice and Zucker diabetic fatty rats in response to
TZDs. Other studies have shown that mouse resistin increases during the differentiation
of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic
differentiation is likely to be associated with a down regulation of resistin gene expression.
Areas of investigation: Insulin resistance, Obesity

Product References (17)

References

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  • Castillero E, Martín AI, Nieto-Bona MP, Fernández-Galaz C, López-Menduiña M,Villanúa MÁ, López-Calderón A. Fenofibrate administration to arthritic ratsincreases adiponectin and leptin and prevents oxidative muscle wasting. EndocrConnect. 2012 Jun 8;1(1):1-12. doi: 10.1530/EC-12-0003. Print 2012 Jul 1. PubMed PMID: 23781298; PubMed Central PMCID: PMC3681315. See more on PubMed
  • Chabrolle C, Jeanpierre E, Tosca L, Ramé C, Dupont J. Effects of high levelsof glucose on the steroidogenesis and the expression of adiponectin receptors in rat ovarian cells. Reprod Biol Endocrinol. 2008 Mar 19;6:11. doi:10.1186/1477-7827-6-11. PubMed PMID: 18353182; PubMed Central PMCID: PMC2277425. See more on PubMed
  • Escrivá F, Gavete ML, Fermín Y, Pérez C, Gallardo N, Alvarez C, Andrés A, Ros M, Carrascosa JM. Effect of age and moderate food restriction on insulinsensitivity in Wistar rats: role of adiposity. J Endocrinol. 2007Jul;194(1):131-41. PubMed PMID: 17592027. See more on PubMed
  • Fernández CM, Moltó E, Gallardo N, del Arco A, Martínez C, Andrés A, Ros M,Carrascosa JM, Arribas C. The expression of rat resistin isoforms isdifferentially regulated in visceral adipose tissues: effects of aging and foodrestriction. Metabolism. 2009 Feb;58(2):204-11. doi:10.1016/j.metabol.2008.09.014. PubMed PMID: 19154953. See more on PubMed
  • Jeyapal S, Kona SR, Mullapudi SV, Putcha UK, Gurumurthy P, Ibrahim A.Substitution of linoleic acid with α-linolenic acid or long chain n-3polyunsaturated fatty acid prevents Western diet induced nonalcoholicsteatohepatitis. Sci Rep. 2018 Jul 19;8(1):10953. doi:10.1038/s41598-018-29222-y. PubMed PMID: 30026586; PubMed Central PMCID:PMC6053361. See more on PubMed
  • Mannerås L, Fazliana M, Wan Nazaimoon WM, Lönn M, Gu HF, Ostenson CG,Stener-Victorin E. Beneficial metabolic effects of the Malaysian herb Labisiapumila var. alata in a rat model of polycystic ovary syndrome. J Ethnopharmacol. 2010 Feb 3;127(2):346-51. doi: 10.1016/j.jep.2009.10.032. Epub 2009 Oct 31.PubMed PMID: 19883744. See more on PubMed
  • Rodríguez M, Pintado C, Moltó E, Gallardo N, Fernández-Martos CM, López V,Andrés A, Arribas C. Central s-resistin deficiency ameliorates hypothalamicinflammation and increases whole body insulin sensitivity. Sci Rep. 2018 Mar2;8(1):3921. doi: 10.1038/s41598-018-22255-3. PubMed PMID: 29500410; PubMedCentral PMCID: PMC5834531. See more on PubMed
  • Sassek M, Pruszynska-Oszmalek E, Kołodziejski PA, Szczepankiewicz D, KaczmarekP, Wieloch M, Kurto K, Nogowski L, Nowak KW, Strowski MZ, Mackowiak P. Resistinis produced by rat pancreatic islets and regulates insulin and glucagon in vitro secretion. Islets. 2016 Nov;8(6):177-185. Epub 2016 Oct 31. PubMed PMID:27797297; PubMed Central PMCID: PMC5161143. See more on PubMed
  • Shirvani H, Rahmati-Ahmadabad S. Irisin interaction with adipose tissuesecretions by exercise training and flaxseed oil supplement. Lipids Health Dis.2019 Jan 17;18(1):15. doi: 10.1186/s12944-019-0960-4. PubMed PMID: 30654813;PubMed Central PMCID: PMC6337839. See more on PubMed
  • Stroubini T, Perelas A, Liapi C, Perrea D, Dontas I, Tzavara Ch, Galanopoulou P. Serum adiponectin and resistin in rats under three isocaloric diets: Theeffect of sibutramine. Cytokine. 2009 May;46(2):171-5. doi:10.1016/j.cyto.2009.01.003. Epub 2009 Feb 28. PubMed PMID: 19251435. See more on PubMed
  • Tatsumi K, Sasaki H, Fujita A, Doi A, Kanaya Y, Furuta H, Nishi M, Tsuno T,Taniguchi H, Nanjo K. Effect of anti-oxidants, Ricetrienol and α-tocopherol, onadipocytokine abnormalities and fatty liver in Otsuka Long-Evans Tokushima Fatty diabetic rats. J Diabetes Investig. 2011 Jun 5;2(3):186-92. doi:10.1111/j.2040-1124.2010.00090.x. PubMed PMID: 24843482; PubMed Central PMCID:PMC4014917. See more on PubMed
  • Chou CL, Lin H, Chen JS, Fang TC. Renin inhibition improves metabolicsyndrome, and reduces angiotensin II levels and oxidative stress in visceral fat tissues in fructose-fed rats. PLoS One. 2017 Jul 10;12(7):e0180712. doi:10.1371/journal.pone.0180712. eCollection 2017. PubMed PMID: 28700686; PubMedCentral PMCID: PMC5507254. See more on PubMed
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Summary References (21)

References to Resistin

  • Banerjee RR, Lazar MA. Dimerization of resistin and resistin-like molecules is determined by a single cysteine. J Biol Chem. 2001 Jul 13;276 (28):25970-3
  • Bokarewa M, Nagaev I, Dahlberg L, Smith U, Tarkowski A. Resistin, an adipokine with potent proinflammatory properties. J Immunol. 2005 May 1;174 (9):5789-95
  • Fasshauer M, Klein J, Neumann S, Eszlinger M, Paschke R. Tumor necrosis factor alpha is a negative regulator of resistin gene expression and secretion in 3T3-L1 adipocytes. Biochem Biophys Res Commun. 2001 Nov 9;288 (4):1027-31
  • Fasshauer M, Paschke R. Regulation of adipocytokines and insulin resistance. Diabetologia. 2003 Dec;46 (12):1594-603
  • Gong H, Ni Y, Guo X, Fei L, Pan X, Guo M, Chen R. Resistin promotes 3T3-L1 preadipocyte differentiation. Eur J Endocrinol. 2004 Jun;150 (6):885-92
  • Hartman HB, Hu X, Tyler KX, Dalal CK, Lazar MA:. Mechanisms Regulating Adipocyte Expression of Resistin. J Biol Chem. 277, 19754-19761, (2002) .
  • Juan CC, Au LC, Fang VS, Kang SF, Ko YH, Kuo SF, Hsu YP, Kwok CF, Ho LT. Suppressed gene expression of adipocyte resistin in an insulin-resistant rat model probably by elevated free fatty acids. Biochem Biophys Res Commun. 2001 Dec 21;289 (5):1328-33
  • Kershaw EE, Flier JS. Adipose tissue as an endocrine organ. J Clin Endocrinol Metab. 2004 Jun;89 (6):2548-56
  • Kim KH, Lee K, Moon YS, Sul HS. A cysteine-rich adipose tissue-specific secretory factor inhibits adipocyte differentiation. J Biol Chem. 2001 Apr 6;276 (14):11252-6
  • Maebuchi M, Machidori M, Urade R, Ogawa T, Moriyama T:. Low resistin levels in adipose tissues and serum high-fat fed mice and genetically obese mice: development of an ELISA system for quantification of resistin. Archiv Biochem Biophys. 416, 164-170 (2003) .
  • Malo E, Ukkola O, Jokela M, Moilanen L, Kahonen M, Nieminen MS, Salomaa V, Jula A, Kesaniemi YA. Resistin is an indicator of the metabolic syndrome according to five different definitions in the Finnish Health 2000 survey. Metab Syndr Relat Disord. 2011 Jun;9 (3):203-10
  • Meier U, Gressner AM. Endocrine regulation of energy metabolism: review of pathobiochemical and clinical chemical aspects of leptin, ghrelin, adiponectin, and resistin. Clin Chem. 2004 Sep;50 (9):1511-25
  • Milan G, Granzotto M, Scarda A, Calcagno A, Pagano C, Federspil G, Vettor R. Resistin and adiponectin expression in visceral fat of obese rats: effect of weight loss. Obes Res. 2002 Nov;10 (11):1095-103
  • Nogueiras R, Gallego R, Gualillo O, Caminos JE, Garcia-Caballero T, Casanueva FF, Dieguez C. Resistin is expressed in different rat tissues and is regulated in a tissue- and gender-specific manner. FEBS Lett. 2003 Jul 31;548 (1-3):21-7
  • Pravenec M, Kazdová L, Landa V, Zídek V, Mlejnek P, Jansa P, Wang J, Qi N, Kurtz TW:. Transgenic and recombinant resistin impair skeletal muscle glucose metabolism in the spontaneously hypertensive rat. J Biol Chem. 278, 45209-45215, (2001) .
  • Rajala MW, Obici S, Scherer PE, Rossetti L. Adipose-derived resistin and gut-derived resistin-like molecule-beta selectively impair insulin action on glucose production. J Clin Invest. 2003 Jan;111 (2):225-30
  • Sadashiv, Tiwari S, Paul BN, Kumar S, Chandra A, Dhananjai S, Negi MP. Over expression of resistin in adipose tissue of the obese induces insulin resistance. World J Diabetes. 2012 Jul 15;3 (7):135-41
  • Satoh H, Nguyen MT, Miles PD, Imamura T, Usui I, Olefsky JM. Adenovirus-mediated chronic "hyper-resistinemia" leads to in vivo insulin resistance in normal rats. J Clin Invest. 2004 Jul;114 (2):224-31
  • Steppan CM, Bailley ST, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, Lazar MA:. The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001) .
  • Steppan CM, Brown EJ, Wright CM, Bhat S, Banerjee RR, Dai CY, Enders GH, Silberg DG, Wen X, Wu GD, Lazar MA:. A Family of Tissue-specific Resistin-like Molecules. PNAS. 98, 502-506, (2001) .
  • Steppan CM, Lazar MA. The current biology of resistin. J Intern Med. 2004 Apr;255 (4):
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