Sandwich ELISA, Biotin-labelled antibody
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Limit of Detection
Analytical Limit of Detection is calculated from the real Resistin values in wells and is 0.05ng/ml
n = 8; CV = 5.1%
n = 5; CV = 7.1%
- bovine Non-detectable
- cat Non-detectable
- dog Non-detectable
- hamster Non-detectable
- horse Non-detectable
- goat Yes
- monkey Non-detectable
- mouse Non-detectable
- pig Non-detectable
- rabbit Non-detectable
- rat Non-detectable
- sheep Non-detectable
- human Non-detectable
- chicken Not tested
- It is intended for research use only
- The total assay time is less than 4 hours
- The kit measures total serum (homodimeric) resistin
- Assay format is 96 wells
- Quality Controls are recombinant rat resistin based
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Animal studies
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of
cysteine-rich secreted proteins which is termed the RELM family, and is also described as
ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone).
Rat resistin contains 114 amino acids as a prepeptide, and its hydrofobic signal peptide is
cleaved before its secretion. Resistin circulates in rat blood as a dimeric protein consisting of
two 96 amino acid polypeptides, which are disulfide-linked.
Resistin may be an important link between obesity and insulin resistance. Mouse resistin,
specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes,
hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan
et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake.
They have also suggested that resistin is present at elevated levels in blood of obese mice,
and is down regulated by fasting and antidiabetic drugs (TZDs). On the other hand, several
studies demonstrated reduced resistin expression in adipose tissue of obese mice and
increased levels in leptin deficient ob/ob mice and Zucker diabetic fatty rats in response to
TZDs. Other studies have shown that mouse resistin increases during the differentiation
of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic
differentiation is likely to be associated with a down regulation of resistin gene expression.
Areas of investigation: Insulin resistance, Obesity