Total 204 AA. MW: 23 kDa (calculated). N-terminal His-tag, 21 extra AA (highlighted). The AA sequence is identical to UniProtKB/Swiss-Prot entry Q5VY30 (AA16–199).
Amino Acid Sequence
> 95%, as determined by SDS-PAGE.
12% SDS-PAGE separation of Human RBP4
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 5μg / lane
3. non-reduced and non-heated sample, 5μg / lane
Determined by its ability to bind all trans retinol acids. The binding of RBP4 to all trans retinol acids results in quenching of Trp fluroscence. 0.5 M retinol acids/per M RBP4.
< 0.1 EU/μg
Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in PBS buffer, pH 7.5
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
Western blotting, ELISA, Binding assay
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
This product is intended for research use only.