SARS-CoV-2 Direct LAMP 96-kit

Features

• SARS-CoV-2 Direct LAMP 96-kit allows the isolation free detection of viral nucleic acid
• The total assay time is less than 45 min
• The kit detects SARS-CoV-2 virus nucleic acid from nasopharyngeal swabs collected into non-inactivating Viral Transport Media (VTM)
• The kit capacity is 96 reactions
• Components of the kit are provided ready to use

Research topic

Immune Response, Infection and Inflammation, COVID-19

Summary

The SARS-CoV-2 Direct LAMP 96-kit is an in vitro test kit for the detection of SARS-CoV-2 RNA based on the principle of amplification of viral RNA under isothermal conditions. The reaction takes place in a single tube at constant temperature and uses the principle of the Reverse Transcription Loop Mediated Isothermal Amplifica-tion (RT-LAMP). The input material for the LAMP reaction can be directly the sample medium from the collection tube without the need viral RNA isolation.
In the LAMP reaction, the target sequence is amplified at constant temperature using a set of pri-mers and a fluorescently labelled displaceable probe, in the presence of a polymer-ase which, in addition to replication activity, also has the strand dissociation ability.
The presence of loop primers increases the rate and dynamics of the reaction, so the amount of nucleic acid generated in LAMP reactions tends to be higher than in classical thermocyclic amplification, and detection of the target sequence may be more than half faster.
Detection of the presence of target sequence is mediated by the fluorescence elevation produced by isothermal amplification on an optional plat-form detecting the FAM channel (480-530 nm).