SARS-CoV-2 Direct MULTILAMP 96-kit

Features

• SARS-CoV-2 Direct MULTILAMP 96-kit allows the isolation free detection of viral nucleic acid
• The total assay time is less than 40 min
• The kit detects SARS-CoV-2 virus nucleic acid from nasal swab collected in a suitable inactivation medium
• The kit capacity is 96 reactions
• Components of the kit are provided ready to use
• The kit is compatible with most common real time qPCR cyclers

Research topic

Immune Response, Infection and Inflammation, COVID-19

Summary

The SARS-CoV-2 Direct MULTILAMP 96-kit is based on the principle of reverse transcription LAMP (Reverse Transcription Loop Mediated Isothermal Amplification). LAMP is an innovative genetic testing method applicable directly to the primary samples, resp. swabs, collected in a suitable inactivation medium. The precise primer design allows multiplex amplification of two targets - SARS-CoV-2 RNA S gene and human DNA RNase P gene. The target sequence is amplified by an isothermal polymerase with replication and dissociation activity, fluorescently labelled probes, and loop primers, so the dynamics of the reaction and the amount of DNA copies significantly increase. Amplification is visualised by the fluorescence signal, FAM dye for SARS-CoV-2 and Cy5 dye for RNase P. The use of the SARS-CoV-2 Direct MULTILAMP 96-kit offers advantages over conventional molecular diagnostic methods. All reagents are in one tube, no nucleic acids isolation is needed, so handling time is reduced. Primers are designed for use in multiplex amplification without secondary structures formation. Compatible with all SARS-CoV-2 mutations.