Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

Secretoglobin 3A2 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Pneumo secretory protein 1, PnSP-1, Uteroglobin-related protein 1, SCGB3A2, Secretoglobin 3A2
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price


RD191373200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Bronchoalveolar lavage, Amniotic fluid, Plasma (EDTA, citrate, heparin)

Sample Requirements

35 µl

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2 – 8 °C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

31.3 – 2000 pg/ml

Limit of Detection

12 pg/ml

Intra-assay (Within-Run)

n = 8; CV = 2.3 %

Inter-assay (Run-to-Run)

n = 6; CV = 10.8 %

Spiking Recovery

100.3 %

Dilutation Linearity

104.0 %

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3 hours
  • The kit measures SCGB3A2 in human serum, EDTA plasma, citrate plasma, heparin plasma, amniotic fluid and bronchoalveolar lavage fluid
  • Assay format is 96 wells
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Immune Response, Infection and Inflammation, Oncology, Pulmonary diseases

Summary

Secretoglobin 3A2 (Secretoglobin family 3A member 2; gene scgb3A2; synonym: uteroglobin-related protein 1, UGRP1) is a homodimeric secretory protein thought to play a role in the modulation of inflammation and tumorigenesis. The human SCGB3A2 gene encodes a protein of molecular weight 10.2 kDa consisting of 93 amino acids, of which the N-terminal 21 amino acid residues exhibit a characteristic signal sequence that may function to target the protein to a secretory pathway.
SCGB3A2 is a member of secretoglobin superfamily, a family of small, secreted proteins found in animals exclusively of mammalian lineage. There are 11 human SCGB genes and five pseudogenes.
SCGB3A2 mRNA was shown to be predominantly expressed in the lung (predominantly in club cells), with low levels of expression in the thyroid. SCGB3A2 has been demonstrated as a novel growth factor accelerating lung development during both early and late developmental stages.
Screening for sequence variations in the exons and the 5´ promoter region of the SCGB3A2 gene among patients with asthma and healthy control individuals revealed the presence of a point mutation, in the promoter region, that appears to be responsible for reduced transcriptional activity of the gene and is associated with an increased risk of asthma.
Model mice for allergic airway inflammation were intranasally administered recombinant adenovirus that express SCGB3A2, and the effect of overexpressed SCGB3A2 on antigen-induced airway inflammation was investigated. The results provide evidence that up-regulation of SCGB3A2 in the airways can suppress allergic airway inflammation. In the mouse model, recombinant human SCGB3A2 exhibited anti-fibrotic and growth factor activities comparable to those of recombinant mouse SCGB3A2. These results suggest that SCGB3A2 may have a potential to be used as a therapeutic agent in treating patients suffering from lung diseases or promoting lung development in premature infants in future.
SCGB3A2 protein, unlike club cell protein, increases in sputum in asthma and rhinitis. SCGB3A2 mRNA expression was shown to be induced by Th1 cytokine, but suppressed by proinflammatory and Th2 cytokines. SCGBs mRNA expression was altered in chronic rhinosinusitis; particularly, SCGB3A2 protein and mRNA expression was markedly decreased in chronic rhinosinusitis with or without nasal polyps.
A recent meta-analysis indicated a significant correlation between SCGB3A -112G/A polymorphism and asthma for AA versus GG (P=0.01) and AA versus GA/GG (P=0.02). Stratification by ethnicity confirmed this result only in Asians but not in Caucasians in the same study. Polymorphism of the SCGB3A2 gene thus may contribute to asthma risk, particularly in Asian population.
The expression of SCGB3A2 was reported in human carcinomas, suggesting the use of this protein as a tumour marker. SCGB3A2 normally expressed in pulmonary club cells, was found negative in Type II cell hyperplasias and adenomas. However, it was expressed in alveolar Type II cell carcinomas and club cell adenocarcinomas. SCGB3A2 has been shown as a potentially useful marker for diagnosis of pulmonary tumours both in mice and humans.

References to Summary

References to Secretoglobin Family 3A Member 2

  • Cai Y, Winn ME, Zehmer JK, Gillette WK, Lubkowski JT, Pilon AL, Kimura S. Preclinical evaluation of human secretoglobin 3A2 in mouse models of lung development and fibrosis. Am J Physiol Lung Cell Mol Phy. 2014 Jan 1;306 (1):L10-22
  • Chiba Y, Kurotani R, Kusakabe T, Miura T, Link BW, Misawa M, Kimura S. Uteroglobin-related protein 1 expression suppresses allergic airway inflammation in mice. Am J Respir Crit Care Med. 2006 May 1;173 (9):958-64
  • de Burbure C, Pignatti P, Corradi M, Malerba M, Clippe A, Dumont X, Moscato G, Mutti A, Bernard A. Uteroglobin-related protein 1 and clara cell protein in induced sputum of patients with asthma and rhinitis. Chest. 2007 Jan;131 (1):172-9
  • Jackson BC, Thompson DC, Wright MW, McAndrews M, Bernard A, Nebert DW, Vasiliou V. Update of the human secretoglobin (SCGB) gene superfamily and an example of 'evolutionary bloom' of androgen-binding protein genes within the mouse Scgb gene superfamily. Hum Genomics. 2011 Oct;5 (6):691-702
  • Kurotani R, Kumaki N, Naizhen X, Ward JM, Linnoila RI, Kimura S. Secretoglobin 3A2/uteroglobin-related protein 1 is a novel marker for pulmonary carcinoma in mice and humans. Lung Cancer. 2011 Jan;71 (1):42-8
  • Kurotani R, Tomita T, Yang Q, Carlson BA, Chen C, Kimura S. Role of secretoglobin 3A2 in lung development. Am J Respir Crit Care Med. 2008 Aug 15;178 (4):389-98
  • Lu X, Wang N, Long XB, You XJ, Cui YH, Liu Z. The cytokine-driven regulation of secretoglobins in normal human upper airway and their expression, particularly that of uteroglobin-related protein 1, in chronic rhinosinusitis. Respir Res. 2011 Mar 08;12:28
  • Niimi T, Keck-Waggoner CL, Popescu NC, Zhou Y, Levitt RC, Kimura S. UGRP1, a uteroglobin/Clara cell secretory protein-related protein, is a novel lung-enriched downstream target gene for the T/EBP/NKX2.1 homeodomain transcription factor. Mol Endocrinol. 2001 Nov;15 (11):2021-36
  • Niimi T, Munakata M, Keck-Waggoner CL, Popescu NC, Levitt RC, Hisada M, Kimura S. A polymorphism in the human UGRP1 gene promoter that regulates transcription is associated with an increased risk of asthma. Am J Hum Genet. 2002 Mar;70 (3):718-25
  • Tachihara-Yoshikawa M, Ishida T, Watanabe K, Sugawara A, Kanazawa K, Kanno R, Suzuki T, Niimi T, Kimura S, Munakata M. Expression of secretoglobin3A2 (SCGB3A2) in primary pulmonary carcinomas. Fukushima J Med Sci. 2008 Dec;54 (2):61-72
  • Xie H, Wu M, Shen B, Niu Y, Huo Y, Cheng Y. Association between the -112G/A polymorphism of uteroglobulin-related protein 1 gene and asthma risk: A meta-analysis. Exp Ther Med. 2014 Mar;7 (3):721-727
Related Products Documents