Total 341 AA, UniProt Q6FHJ7 (Val19-Val346). MW: 39.0 kDa (calculated), migrates at ~ 55 kDa on SDS PAGE; two N-terminal linkers (2 extra AA and 3 extra AA), N-terminal Flag-tag (8 extra AA). Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
˃ 90 % by SDS-PAGE
12% SDS-PAGE separation of Human sFRP4
1. M.W. marker – 97, 66, 45, 31, 21, 14 kDa
2. reduced and boiled sample, 2.5μg / lane
3. non-reduced and non-boiled sample, 2.5μg / lane
< 1.0 EU/µg
Filtered (0.4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4
Add 200ul of deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely.
Western blotting, ELISA
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at -80 °C. Lyophilized protein remains stable until the expiry date when stored at -80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein. LAL to determine quantity of endotoxin.
This product is intended for research use only.