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Manufactured by BioVendor

sICAM-2 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Intercellular adhesion molecule 2, CD102 antigen
  • Species:Human
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Cat. No. Size Price


RAF104R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Urine, Amniotic fluid, Plasma, Cell culture supernatant, Whole blood

Sample Requirements

100 µl (1:20 prediluted)

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.75–24 U/ml

Limit of Detection

0.30 U/ml

Intra-assay (Within-Run)

CV = 6.7%

Inter-assay (Run-to-Run)

CV = 7.0%

Summary

Research topic

Cell adhesion proteins

Summary

Intercellular Adhesion Molecule-2 (ICAM-2) is a 60 kDa surface glycoprotein that promotes adhesion between immune cells and the vascular endothelium. ICAM-2 is a member of the Ig-superfamily with two immunoglobulin-like extracellular domains. ICAM-2 is much more closely related to the two N-terminal domains of ICAM-1 (34% identity) than either ICAM-1 or ICAM-2 is to other members of the Ig-superfamily,
demonstrating the existence of a subfamily of Ig-like ligands that bind the same integrin receptor. ICAM-2 is broadly distributed on hematopoietic cells. On resting lymphocytes, the ICAM-2 expression is several-fold higher than that seen with ICAM-1, while monocytes express equivalent levels of ICAM-1 and ICAM-2. In contrast to ICAM-1, neutrophils stain negative for ICAM-2. Just little or no ICAM-2 staining can be demonstrated on other cell lines, the sole exception being (i) resting vascular endothelial cells, which possess high levels of ICAM-2,
and (ii) small clusters of cells in lymphoid tissue germinal centres. ICAM-2 expression is unaffected by a variety of inflammatory cytokines, while ICAM-1 is upregulated upon stimulation. This points toward the importance of ICAM-2 in the unstimulated resting state, before ICAM-1 expression is increased.
The integrin LFA-1 is the receptor for ICAM-2 and ICAM-1 as well. The functional characteristics of ICAM-2 as LFA-1 ligand can be summarized as follows:
ICAM-2 dominates over ICAM-1 on resting endothelial cells and seems to be involved in the recirculation of LFA-1 positive lymphocytes, e.g. facilitating T-memory cell recirculation. As resting T-cells express little or
no ICAM-1, ICAM-2 may also be important in initial T-cell adhesion with antigen-presenting cells that bear LFA-1. Also the lysis of certain target cells appears to occur in an ICAM-1 independent manner, possibly regulated by ICAM-2.

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