Total 331 AA, MW: 35.2 kDa (calculated). C-terminal Flag-tag 11 AA (highlighted).
Amino Acid Sequence
Purity as determined by densitometric image analysis: >95%
12% SDS-PAGE separation of Human sRAGE
1. MW marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 2.5 μg / lane
3. nonreduced and nonheated sample, 2.5 μg / lane
< 1.0 EU/ug
Filtered (0.4 μm) and lyophilized from 0.5 mg/mL in 20 mM Tris, 50 mM NaCl, pH 7.5
Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
Western blotting, ELISA, Cell culture and/or animal studies
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.