Total 141 AA. MW: 16.4 kDa (calculated). UniProtKB acc.no. Q9UNK4. N-Terminal His-tag, 16 extra AA (highlighted).
Amino Acid Sequence
Purity as determined by densitometric image analysis: >95%
12% SDS-PAGE separation of Human sPLA2-IID
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 5μg/lane
3. non-reduced and non-heated sample, 5μg/lane
< 0.1 EU/μg
Filtered (0.4 μm) and frozen from 0.3 – 0.8 mg/mL solution in 0.03M Acetate buffer pH=4.0 containing 20% (w/v) glycerol.
Defrost at ambient temperature. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
On ice. Upon receipt, store the product at the temperature recommended below.
Store protein at –80°C. Protein remains stable until the expiry date when stored at –80°C. Avoid repeated freezing/thawing cycles. The thawed protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
This product is intended for research use only.