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Manufactured by BioVendor

sPLA2-XII Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:Group XIIA secretory phospholipase A2, GXII sPLA2, Phosphatidylcholine 2-acylhydrolase 12A, PLA2G12A, PLA2G12, FKSG38, UNQ2519/PRO6012
  • Species:Human
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Cat. No. Size Price


RD172066100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Total 183 AA. MW: 20.6 kDa (calculated). UniProtKB acc.no. Q9BZM1. N-Terminal His-tag, 16 extra AA (highlighted).

Amino Acid Sequence

MRGSHHHHHHGMASHMQEQAQTTDWRATLKTIRNGVHKIDTYLNAALDLLGGEDGLCQYKCSDGSKPFPRYGYKPSPPNGCGSPLFGVHLNIGIPSLTKCCNQHDRCYETCGKSKNDCDEEFQYCLSKICRDVQKTLGLTQHVQACETTVELLFDSVIHLGCKPYLDSQRAACRCHYEEKTDL

Source

E. coli

Purity

>95%

SDS-PAGE Gel

12% SDS-PAGE separation of Human sPLA2-XI
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 5μg/lane
3. non-reduced and non-heated sample, 5μg/lane

Endotoxin

< 1.0 EU/ug

Formulation

Filtered (0.4 μm) and lyophilized from 0.5 mg/mL in 20mM Tris buffer, 50 mM NaCl, pH 8.6

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Applications

Western blotting

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Physical Appearance

White lyophilized (freeze-dried) powder.

Note

This product is intended for research use only.

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