Sandwich ELISA, Biotin-labelled antibody
Serum, Urine, Milk, Plasma
Serum, Plasma and Urine: 35 µl/well
Breast milk: 5 µl/well
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the complete kit at 2 8°C. Under these conditions, the kit is stable until the expiration date (see label on the box)
15.6 – 1000 pg/ml
Limit of Detection
n = 8; CV = 2.8 %
n = 7; CV = 5.4 %
- It is intended for research use only
- The total assay time is less than 3 hours
- The kit measures STC1 in serum, plasma (EDTA, citrate, heparin), breast milk and urine
- Assay format is 96 wells
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Bone and cartilage metabolism, Oncology, Reproduction
Stanniocalcin 1 is a glycoprotein, which was originally identified as a calcium/phosphate-regulating hormone in bony fishes. The gen STC-1 encodes a 247-amino acids protein that exists as a homodimer and is secreted into the extracellular space owing to a signal peptide sequence.
STC-1 is expressed in a wide variety of tissues, including heart, kidney, ovary, prostate, stomach and thyroid and may have autocrine or paracrine functions. Current research indicates that Stanniocalcin 1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and was found to be a selective modulator of hepatocyte growth factor (HGF). Current research indicates that induced endothelial migration and morphogenesis, an inhibitor of macrophage chemotaxis and chemokinesis, suppressor of progesterone and luteinization inhibitor. Together with STC2, it may play important roles in the processes of implantation and decidualization. In terminally differentiated adipocytes, it may function as a "survival factor", which contributes to the maintenance of the integrity of mature adipose tissue. This protein also may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. The results sugested that might be a useful therapy for optic nerve diseases and protects retinal ganglion cells by inhibiting apoptosis and oxidative demage.
STC1 can affect bone and muscle mass and structure, and angiogenesis through effects on osteoblasts, osteoclasts, myoblasts/myocytes, and endothelial cells in mouse model. Differential regulation of myocardial STC1 protein expression was reported in heart failure. In addition, STC1 may regulate calcium currents in cardiomyocytes and may contribute to the alterations in calcium homeostasis of the failing heart. Stanniocalcin 1 is differentially expressed in the culprit coronary plaques of patients with acute myocardial infarction versus those with stable angina. STC1 may play a role in plaque instability.
STC1 is involved in various physiological processes, such as ion transport, reproduction and development. Their expression in the uterus and roles in implantation and early pregnancy are unclear. During pregnancy and lactation in rodents, stanniocalcin 1 production by the ovaries is upregulated markedly and released into the circulation. Serum progesterone levels are low during lactation, when serum levels of STC1 are correspondingly elevated.
Most studies suggests that STC-1 gene has altered expression in ovarian, breast and lung cancers and is a new biomarker of glioma progression. In addition Stanniocalcin 1 upregulation play an important role in gastric cancer development and serum STC1 might function as promising tumor marker for gastric cancer diagnosis and prognosis. STC1 is also involved in the lactation process and may be implicated in the control of involution. It was shown that STC1 in milk is increased following milk stasis.