Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

Stanniocalcin 1 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:STC1, STC
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price


RD191095200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Urine, Milk, Plasma (EDTA, citrate, heparin)

Sample Requirements

Serum, Plasma and Urine: 35 µl/well
Breast milk: 5 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2 8°C. Under these conditions, the kit is stable until the expiration date (see label on the box)

Calibration Curve

Calibration Range

15.6 – 1000 pg/ml

Limit of Detection

0.7 pg/ml

Intra-assay (Within-Run)

n = 8; CV = 2.8 %

Inter-assay (Run-to-Run)

n = 7; CV = 5.4 %

Spiking Recovery

100.0 %

Dilutation Linearity

99.2 %

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3 hours
  • The kit measures STC1 in serum, plasma (EDTA, citrate, heparin), breast milk and urine
  • Assay format is 96 wells
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Bone and cartilage metabolism, Oncology, Reproduction

Summary

Stanniocalcin 1 is a glycoprotein, which was originally identified as a calcium/phosphate-regulating hormone in bony fishes. The gen STC-1 encodes a 247-amino acids protein that exists as a homodimer and is secreted into the extracellular space owing to a signal peptide sequence.
STC-1 is expressed in a wide variety of tissues, including heart, kidney, ovary, prostate, stomach and thyroid and may have autocrine or paracrine functions. Current research indicates that Stanniocalcin 1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and was found to be a selective modulator of hepatocyte growth factor (HGF). Current research indicates that induced endothelial migration and morphogenesis, an inhibitor of macrophage chemotaxis and chemokinesis, suppressor of progesterone and luteinization inhibitor. Together with STC2, it may play important roles in the processes of implantation and decidualization. In terminally differentiated adipocytes, it may function as a "survival factor", which contributes to the maintenance of the integrity of mature adipose tissue. This protein also may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. The results sugested that might be a useful therapy for optic nerve diseases and protects retinal ganglion cells by inhibiting apoptosis and oxidative demage.
STC1 can affect bone and muscle mass and structure, and angiogenesis through effects on osteoblasts, osteoclasts, myoblasts/myocytes, and endothelial cells in mouse model. Differential regulation of myocardial STC1 protein expression was reported in heart failure. In addition, STC1 may regulate calcium currents in cardiomyocytes and may contribute to the alterations in calcium homeostasis of the failing heart. Stanniocalcin 1 is differentially expressed in the culprit coronary plaques of patients with acute myocardial infarction versus those with stable angina. STC1 may play a role in plaque instability.
STC1 is involved in various physiological processes, such as ion transport, reproduction and development. Their expression in the uterus and roles in implantation and early pregnancy are unclear. During pregnancy and lactation in rodents, stanniocalcin 1 production by the ovaries is upregulated markedly and released into the circulation. Serum progesterone levels are low during lactation, when serum levels of STC1 are correspondingly elevated.
Most studies suggests that STC-1 gene has altered expression in ovarian, breast and lung cancers and is a new biomarker of glioma progression. In addition Stanniocalcin 1 upregulation play an important role in gastric cancer development and serum STC1 might function as promising tumor marker for gastric cancer diagnosis and prognosis. STC1 is also involved in the lactation process and may be implicated in the control of involution. It was shown that STC1 in milk is increased following milk stasis.

Summary References (18)

References to Stanniocalcin 1

  • Arigami T, Uenosono Y, Ishigami S, Hagihara T, Haraguchi N, Matsushita D, Yanagita S, Nakajo A, Okumura H, Hokita S, Natsugoe S. Expression of stanniocalcin 1 as a potential biomarker of gastric cancer. Oncology. 2012;83 (3):158-64
  • Chang AC, Doherty J, Huschtscha LI, Redvers R, Restall C, Reddel RR, Anderson RL. STC1 expression is associated with tumor growth and metastasis in breast cancer. Clin Exp Metastasis. 2015 Jan;32 (1):15-27
  • Chang AC, Jeffrey KJ, Tokutake Y, Shimamoto A, Neumann AA, Dunham MA, Cha J, Sugawara M, Furuichi Y, Reddel RR. Human stanniocalcin (STC): genomic structure, chromosomal localization, and the presence of CAG trinucleotide repeats. Genomics. 1998 Feb 1;47 (3):393-8
  • dos Santos MT, Trindade DM, Goncalves Kde A, Bressan GC, Anastassopoulos F, Yunes JA, Kobarg J. Human stanniocalcin-1 interacts with nuclear and cytoplasmic proteins and acts as a SUMO E3 ligase. Mol Biosyst. 2011 Jan;7 (1):180-93
  • Du YZ, Gu XH, Li L, Gao F. The diagnostic value of circulating stanniocalcin-1 mRNA in non-small cell lung cancer. J Surg Oncol. 2011 Dec;104 (7):836-40
  • Fang Z, Tian Z, Luo K, Song H, Yi J. Clinical significance of stanniocalcin expression in tissue and serum of gastric cancer patients. Chin J Cancer Res. 2014 Oct;26 (5):602-10
  • Filvaroff EH, Guillet S, Zlot C, Bao M, Ingle G, Steinmetz H, Hoeffel J, Bunting S, Ross J, Carano RA, Powell-Braxton L, Wagner GF, Eckert R, Gerritsen ME, French DM. Stanniocalcin 1 alters muscle and bone structure and function in transgenic mice. Endocrinology. 2002 Sep;143 (9):3681-90
  • Fujiwara Y, Sugita Y, Nakamori S, Miyamoto A, Shiozaki K, Nagano H, Sakon M, Monden M. Assessment of Stanniocalcin-1 mRNA as a molecular marker for micrometastases of various human cancers. Int J Oncol. 2000 Apr;16 (4):799-804
  • Hasilo CP, McCudden CR, Gillespie JR, James KA, Hirvi ER, Zaidi D, Wagner GF. Nuclear targeting of stanniocalcin to mammary gland alveolar cells during pregnancy and lactation. Am J Physiol Endocrinol Metab. 2005 Oct;289 (4):E634-42
  • Hasselblatt M, Bohm C, Tatenhorst L, Dinh V, Newrzella D, Keyvani K, Jeibmann A, Buerger H, Rickert CH, Paulus W. Identification of novel diagnostic markers for choroid plexus tumors: a microarray-based approach. Am J Surg Pathol. 2006 Jan;30 (1):66-74
  • James K, Seitelbach M, McCudden CR, Wagner GF. Evidence for stanniocalcin binding activity in mammalian blood and glomerular filtrate. Kidney Int. 2005 Feb;67 (2):477-82
  • Jiang WQ, Chang AC, Satoh M, Furuichi Y, Tam PP, Reddel RR. The distribution of stanniocalcin 1 protein in fetal mouse tissues suggests a role in bone and muscle development. J Endocrinol. 2000 May;165 (2):457-66
  • Kanellis J, Bick R, Garcia G, Truong L, Tsao CC, Etemadmoghadam D, Poindexter B, Feng L, Johnson RJ, Sheikh-Hamad D. Stanniocalcin-1, an inhibitor of macrophage chemotaxis and chemokinesis. Am J Physiol Renal Physiol. 2004 Feb;286 (2):F356-62
  • Kim SJ, Ko JH, Yun JH, Kim JA, Kim TE, Lee HJ, Kim SH, Park KH, Oh JY. Stanniocalcin-1 protects retinal ganglion cells by inhibiting apoptosis and oxidative damage. PLoS One. 2013;8 (5):e63749
  • Lal A, Peters H, St Croix B, Haroon ZA, Dewhirst MW, Strausberg RL, Kaanders JH, van der Kogel AJ, Riggins GJ. Transcriptional response to hypoxia in human tumors. J Natl Cancer Inst. 2001 Sep 5;93 (17):1337-43
  • Lee CW, Hwang I, Park CS, Lee H, Park DW, Kang SJ, Lee SW, Kim YH, Park SW, Park SJ. Expression of stanniocalcin-1 in culprit coronary plaques of patients with acute myocardial infarction or stable angina. J Clin Pathol. 2013 Sep;66 (9):787-91
  • Liu G, Yang G, Chang B, Mercado-Uribe I, Huang M, Zheng J, Bast RC, Lin SH, Liu J. Stanniocalcin 1 and ovarian tumorigenesis. J Natl Cancer Inst. 2010 Jun 2;102 (11):812-27
  • Madsen KL, Tavernini MM, Yachimec C, Mendrick DL, Alfonso PJ, Buergin M, Olsen HS, Antonaccio MJ, Thomson AB, Fedorak RN. Stanniocalcin: a novel protein regulating calcium and phosphate transport across mammalian intestine. Am J Physi
Related Products Docs