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Manufactured by BioVendor

Surfactant Protein A Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:SP-A
  • Species:Human
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Cat. No. Size Price


RD191139200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-Heparin, Plasma-Citrate, Bronchoalveolar lavage, Amniotic fluid

Sample Requirements

15 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1–50 ng/ml

Limit of Detection

0.16 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 4.0%

Inter-assay (Run-to-Run)

n = 8; CV = 9.5%

Spiking Recovery

98,40%

Dilutation Linearity

102,00%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • monkey Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • rabbit Not tested
  • human Yes
Summary

Features

  • It is intended for research use only
  • The total assay time is less than 4 hours
  • The kit measures total surfactant protein A in serum, plasma (citrate, heparin), bronchoalveolar lavage fluid and amniotic fluid
  • Assay format is 96 wells
  • Quality Controls are human serum based. No animal sera are used
  • Standard is native protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Immune Response, Infection and Inflammation, Pulmonary diseases, Reproduction

Summary

Surfactant protein A (SP-A; surfactant- associated protein A – SFTPA; pulmonary surfactant- associated protein A – PSPA) is the major protein component of the lung surfactant in mammals. It belongs, together with SP-D, to the collectins’ family, due to its C-type lectin domain and the collagen-like N-terminal triple helix. The lectin domain mediates the interaction between a collectin and a wide variety of pathogens. The oligomeric structure of SP-A comprises two types of polypeptide chains: SP-A1 and SP-A2 transcribed from sftpa1 and sftpa2 genes. Six trimers of SP-A form its complete octadecameric structure. Human SP-A is expressed primarily in the lung, mostly in alveolar type II cells and also in non-ciliated bronchial epithelial cells. SP-A mRNA can be detected in fetal lung cells after only 20 weeks of gestation. SP-A has been found in various biological fluids, such as bronchoalveolar lavage fluid, sputum, serum, amniotic fluid and vaginal lavage fluid. It has also been found to be expressed in cells of the small and large intestine. SP-A is considered to be an important host defence components against respiratory allergens and pathogens. SP-A takes part in modulating the function of immune system cells – especially dendritic cells and T-cells. Many studies have shown that inflammatory mediators (e.g. TNF) are controlled by SP-A, both positively and negatively. Changes in SP-A production in the lung or its baseline concentrations in serum might be associated with progression or mortality at idiopatic pulmonary fibrosis (IPF). Reduced levels of SP-A in alveolar fluid were also reported in other pulmonary diseases, e.g. acute respiratory distress syndrome (ARDS). Furthermore, elevated levels of SP-A in lungs have been observed in pulmonary alveolar proteinosis (PAP).

Product References (13)

References

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  • Dy ABC, Arif MZ, Addison KJ, Que LG, Boitano S, Kraft M, Ledford JG. GeneticVariation in Surfactant Protein-A2 Delays Resolution of Eosinophilia in Asthma. JImmunol. 2019 Jul 26. pii: ji1900546. doi: 10.4049/jimmunol.1900546. [Epub ahead of print] PubMed PMID: 31350355. See more on PubMed
  • Emilsson ÖI, Benediktsdóttir B, Ólafsson Í, Cook E, Júlíusson S, Björnsson ES,Guðlaugsdóttir S, Guðmundsdóttir AS, Mirgorodskaya E, Ljungström E, ArnardóttirES, Gíslason T, Janson C, Olin AC. Respiratory symptoms, sleep-disorderedbreathing and biomarkers in nocturnal gastroesophageal reflux. Respir Res. 2016Sep 20;17(1):115. doi: 10.1186/s12931-016-0431-7. Erratum in: Respir Res. 2016Oct 18;17 (1):130. PubMed PMID: 27646537; PubMed Central PMCID: PMC5029098. See more on PubMed
  • Gargiulo P, Banfi C, Ghilardi S, Magrì D, Giovannardi M, Bonomi A, Salvioni E,Battaia E, Filardi PP, Tremoli E, Agostoni P. Surfactant-derived proteins asmarkers of alveolar membrane damage in heart failure. PLoS One. 2014 Dec16;9(12):e115030. doi: 10.1371/journal.pone.0115030. eCollection 2014. PubMedPMID: 25514679; PubMed Central PMCID: PMC4267772. See more on PubMed
  • Gaunsbaek MQ, Kjeldsen AD, Svane-Knudsen V, Henriksen ML, Hansen S. Surfactantproteins A, B, C and D in the human nasal airway: associated with mucosal glands and ciliated epithelium but absent in fluid-phase secretions and mucus. ORL JOtorhinolaryngol Relat Spec. 2014;76(5):288-301. doi: 10.1159/000369143. Epub2014 Dec 10. PubMed PMID: 25500788. See more on PubMed
  • Hüttmann EM, Greulich T, Hattesohl A, Schmid S, Noeske S, Herr C, John G,Jörres RA, Müller B, Vogelmeier C, Koczulla AR. Comparison of two devices and twobreathing patterns for exhaled breath condensate sampling. PLoS One.2011;6(11):e27467. doi: 10.1371/journal.pone.0027467. Epub 2011 Nov 7. Erratumin: PLoS One. 2016;11(3):e0152620. PubMed PMID: 22087323; PubMed Central PMCID:PMC3210176. See more on PubMed
  • Lärstad M, Almstrand AC, Larsson P, Bake B, Larsson S, Ljungström E,Mirgorodskaya E, Olin AC. Surfactant Protein A in Exhaled Endogenous Particles IsDecreased in Chronic Obstructive Pulmonary Disease (COPD) Patients: A PilotStudy. PLoS One. 2015 Dec 11;10(12):e0144463. doi: 10.1371/journal.pone.0144463. eCollection 2015. PubMed PMID: 26656890; PubMed Central PMCID: PMC4676630. See more on PubMed
  • Liu HH, Chen CY, Chen GI, Lee LH, Chen HL. Relationship between indiumexposure and oxidative damage in workers in indium tin oxide production plants.Int Arch Occup Environ Health. 2012 May;85(4):447-53. doi:10.1007/s00420-011-0688-6. Epub 2011 Aug 11. PubMed PMID: 21833746. See more on PubMed
  • Malur A, Kavuru MS, Marshall I, Barna BP, Huizar I, Karnekar R, Thomassen MJ. Rituximab therapy in pulmonary alveolar proteinosis improves alveolar macrophage lipid homeostasis. Respir Res. 2012 Jun 14;13:46. doi: 10.1186/1465-9921-13-46.PubMed PMID: 22697800; PubMed Central PMCID: PMC3432632. See more on PubMed
  • Podolanczuk AJ, Oelsner EC, Barr RG, Hoffman EA, Armstrong HF, Austin JH,Basner RC, Bartels MN, Christie JD, Enright PL, Gochuico BR, Hinckley StukovskyK, Kaufman JD, Hrudaya Nath P, Newell JD Jr, Palmer SM, Rabinowitz D, Raghu G,Sell JL, Sieren J, Sonavane SK, Tracy RP, Watts JR, Williams K, Kawut SM, LedererDJ. High attenuation areas on chest computed tomography in community-dwellingadults: the MESA study. Eur Respir J. 2016 Nov;48(5):1442-1452. doi:10.1183/13993003.00129-2016. Epub 2016 Jul 28. PubMed PMID: 27471206; PubMedCentral PMCID: PMC5089905. See more on PubMed
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  • Susilovic-Grabovac Z, Banfi C, Brusoni D, Mapelli M, Ghilardi S, Obad A,Bakovic-Kramaric D, Dujic Z, Agostoni P. Diving and pulmonary physiology:Surfactant binding protein, lung fluid and cardiopulmonary test changes inprofessional divers. Respir Physiol Neurobiol. 2017 Sep;243:27-31. doi:10.1016/j.resp.2017.04.012. Epub 2017 Apr 30. PubMed PMID: 28467885. See more on PubMed
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Summary References (12)

References to Surfactant Protein A

  • Doyle IR, Bersten AD, Nicholas TE. Surfactant proteins-A and -B are elevated in plasma of patients with acute respiratory failure. Am J Respir Crit Care Med. 1997 Oct;156 (4 Pt 1):1217-29
  • Greene KE, King TE Jr, Kuroki Y, Bucher-Bartelson B, Hunninghake GW, Newman LS, Nagae H, Mason RJ. Serum surfactant proteins-A and -D as biomarkers in idiopathic pulmonary fibrosis. Eur Respir J. 2002 Mar;19 (3):439-46
  • Greene KE, Wright JR, Steinberg KP, Ruzinski JT, Caldwell E, Wong WB, Hull W, Whitsett JA, Akino T, Kuroki Y, Nagae H, Hudson LD, Martin TR. Serial changes in surfactant-associated proteins in lung and serum before and after onset of ARDS. Am J Respir Crit Care Med. 1999 Dec;160 (6):1843-50
  • Gunther A, Schmidt R, Nix F, Yabut-Perez M, Guth C, Rosseau S, Siebert C, Grimminger F, Morr H, Velcovsky HG, Seeger W. Surfactant abnormalities in idiopathic pulmonary fibrosis, hypersensitivity pneumonitis and sarcoidosis. Eur Respir J. 1999 Sep;14 (3):565-73
  • Kinder BW, Brown KK, McCormack FX, Ix JH, Kervitsky A, Schwarz MI, King TE Jr. Serum surfactant protein-A is a strong predictor of early mortality in idiopathic pulmonary fibrosis. Chest. 2009 Jun;135 (6):1557-63
  • Kishore U, Greenhough TJ, Waters P, Shrive AK, Ghai R, Kamran MF, Bernal AL, Reid KB, Madan T, Chakraborty T. Surfactant proteins SP-A and SP-D: structure, function and receptors. Mol Immunol. 2006 Mar;43 (9):1293-315
  • Kuroki Y, Takahashi M, Nishitani C. Pulmonary collectins in innate immunity of the lung. Cell Microbiol. 2007 Aug;9 (8):1871-9
  • Pastva AM, Wright JR, Williams KL. Immunomodulatory roles of surfactant proteins A and D: implications in lung disease. Proc Am Thorac Soc. 2007 Jul;4 (3):252-7
  • Phelps DS, Umstead TM, Mejia M, Carrillo G, Pardo A, Selman M. Increased surfactant protein-A levels in patients with newly diagnosed idiopathic pulmonary fibrosis. Chest. 2004 Feb;125 (2):617-25
  • Sanchez-Barbero F, Rivas G, Steinhilber W, Casals C. Structural and functional differences among human surfactant proteins SP-A1, SP-A2 and co-expressed SP-A1/SP-A2: role of supratrimeric oligomerization. Biochem J. 2007 Sep 15;406 (3):479-89
  • Takahashi H, Fujishima T, Koba H, Murakami S, Kurokawa K, Shibuya Y, Shiratori M, Kuroki Y, Abe S. Serum surfactant proteins A and D as prognostic factors in idiopathic pulmonary fibrosis and their relationship to disease extent. Am J Respir Crit Care Med. 2000 Sep;162 (3 Pt 1):1109-14
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