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Manufactured by BioVendor

Surfactant Protein A Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:SP-A
  • Species:Human
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Cat. No. Size Price

RD191139200R 96 wells (1 kit)
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


Serum, Plasma-Heparin, Plasma-Citrate, Bronchoalveolar lavage, Amniotic fluid, COVID-19

Sample Requirements

15 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1–50 ng/ml

Limit of Detection

0.16 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 4.0%

Inter-assay (Run-to-Run)

n = 8; CV = 9.5%

Spiking Recovery


Dilution Linearity



  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • monkey Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • rabbit Not tested
  • human Yes


  • It is intended for research use only
  • The total assay time is less than 4 hours
  • The kit measures total surfactant protein A in serum, plasma (citrate, heparin), bronchoalveolar lavage fluid and amniotic fluid
  • Assay format is 96 wells
  • Quality Controls are human serum based. No animal sera are used
  • Standard is native protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Immune Response, Infection and Inflammation, Pulmonary diseases, Reproduction, COVID-19


Surfactant protein A (SP-A; surfactant- associated protein A – SFTPA; pulmonary surfactant- associated protein A – PSPA) is the major protein component of the lung surfactant in mammals. It belongs, together with SP-D, to the collectins’ family, due to its C-type lectin domain and the collagen-like N-terminal triple helix. The lectin domain mediates the interaction between a collectin and a wide variety of pathogens. The oligomeric structure of SP-A comprises two types of polypeptide chains: SP-A1 and SP-A2 transcribed from sftpa1 and sftpa2 genes. Six trimers of SP-A form its complete octadecameric structure. Human SP-A is expressed primarily in the lung, mostly in alveolar type II cells and also in non-ciliated bronchial epithelial cells. SP-A mRNA can be detected in fetal lung cells after only 20 weeks of gestation. SP-A has been found in various biological fluids, such as bronchoalveolar lavage fluid, sputum, serum, amniotic fluid and vaginal lavage fluid. It has also been found to be expressed in cells of the small and large intestine. SP-A is considered to be an important host defence components against respiratory allergens and pathogens. SP-A takes part in modulating the function of immune system cells – especially dendritic cells and T-cells. Many studies have shown that inflammatory mediators (e.g. TNF) are controlled by SP-A, both positively and negatively. Changes in SP-A production in the lung or its baseline concentrations in serum might be associated with progression or mortality at idiopatic pulmonary fibrosis (IPF). Reduced levels of SP-A in alveolar fluid were also reported in other pulmonary diseases, e.g. acute respiratory distress syndrome (ARDS). Furthermore, elevated levels of SP-A in lungs have been observed in pulmonary alveolar proteinosis (PAP).

Product References (15)


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Summary References (12)

References to Surfactant Protein A

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  • Kinder BW, Brown KK, McCormack FX, Ix JH, Kervitsky A, Schwarz MI, King TE Jr. Serum surfactant protein-A is a strong predictor of early mortality in idiopathic pulmonary fibrosis. Chest. 2009 Jun;135 (6):1557-63
  • Kishore U, Greenhough TJ, Waters P, Shrive AK, Ghai R, Kamran MF, Bernal AL, Reid KB, Madan T, Chakraborty T. Surfactant proteins SP-A and SP-D: structure, function and receptors. Mol Immunol. 2006 Mar;43 (9):1293-315
  • Kuroki Y, Takahashi M, Nishitani C. Pulmonary collectins in innate immunity of the lung. Cell Microbiol. 2007 Aug;9 (8):1871-9
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