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Manufactured by BioVendor

Testis Fatty Acid Binding Protein Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:Testis lipid-binding protein, TLBP, Fatty acid-binding protein 9, T-FABP, FABP9
  • Species:Human
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Cat. No. Size Price


RD172251100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Total 141 AA. MW: 16.2 kDa (calculated). UniProtKB acc.no. Q0Z7S8. N-Terminal His-tag (10 extra AA)

Amino Acid Sequence

MKHHHHHHASVEPFLGTWKLVSSENFEDYMKELGVNFAARNMAGLVKPTVTISVDGKMMTIRTESSFQDTKISFKLGEEFDETTADNRKVKSTITLENGSMIHVQKWLGKETTIKRKIVDEKMVVECKMNNIVSTRIYEKV

Source

E. coli

Purity

Purity as determined by densitometric image analysis > 99%

SDS-PAGE Gel

14% SDS-PAGE separation of Human FABP9
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and boiled sample, 5μg/lane
3. non-reduced and non-boiled sample, 5μg/lane

Endotoxin

<1.0 EU/ug

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Applications

Western blotting, ELISA

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Summary

Research topic

Reproduction

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