thrombomiR™ kit

Cat. No.	Size	Price
KT-021-TR96	Analysis of 48 samples
KT-021-TR384	Analysis of 48 samples

Technical Data

Type

qPCR miRNA screening panel

Description

The thrombomiR™ kit enables simple and standardized analysis of microRNA biomarkers for platelet function.

The kit contains 10 individual platelet function-related biomarkers for

monitoring of the drug effects on platelet function, reactivity and haemostasis
diagnosis of platelet-related disorders

The kit has the following unique features

Works with frozen sample material (serum or plasma)
Responds to all platelet-activating signals (ADP, Collagen, etc.)
Measures a platelet-signal generated in-vivo thus complementing results from ex vivo platelet-function tests (LTAs, VASP, …)

All-In-One kit: the thrombomiR™ kit contains all necessary reagents for RNA extraction, cDNA synthesis, preparation of PCR Mix, real time PCR analysis, data analysis: tailored software.

Applications

Platelets poor plasma

Sample Requirements

200 μL of PPP

Summary

Features

It is intended for research use only.

Each kit comprises the following components:

Plasma RNA extraction kit containing all reagents, spin columns and collection tubes
The RT-qPCR chemistry comprising spike-in controls, all reagents for cDNA synthesis and the miGreen PCR Mix;
Primer-coated qPCR plates in 96 well or 384 well format

Research topic

Serology

Summary

The thrombomiR® test enables quantitative analysis of 10 platelet microRNAs, 1 liver-specific microRNA (control) and 5 assay and sample quality controls in human platelet-poor plasma (PPP) samples. These microRNAs are novel biomarkers that can supply information about the molecular function of platelets in vivo, determine the effect of a treatment on platelet reactivity, and study the utility of platelet microRNAs as risk factors for cardiovascular and metabolic diseases.

Summary References (8)

References to platelet function - related miRNA

Bye A, et al. Circulating microRNAs predict future fatal myocardial infarction in healthy individuals - The HUNT study. 2016 J Mol Cell Cardiol.
Kaudewitz D, et al. Association of MicroRNAs and YRNAs With Platelet Function. 2016 Circ Res.
Mayr M, et al. MicroRNAs within the continuum of postgenomics biomarker discovery. 2013 Arterioscler Thromb Vasc Biol.
Willeit P, et al. Circulating microRNAs as novel biomarkers for platelet activation. 2013 Circ Res.
Willeit P, et al. Circulating MicroRNA-122 Is Associated With The Risk of New-Onset Metabolic Syndrome And Type-2-Diabetes. 2016 Diabetes.
Zampetaki A, et al. Prospective study on circulating MicroRNAs and risk of myocardial infarction. 2012 J Am Coll Cardiol.
Zampetaki A, et al. Plasma microRNA profling reveals loss of endothelial miR-126 and other microRNAs in type 2 diabetes. 2010 Circ Res.
Sunderland N, et al. MicroRNA Biomarkers and Platelet Reactivity: The Clot Thickens. 2017 Circ. Res.

Docs

Product Datasheet (RUO)

Datasheet PDF (RUO)

Product Brochure

Product description