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Tigar Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:TP53-induced glycolysis and apoptosis regulator (TIGAR)
  • Species:Human
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Cat. No. Size Price


RBG10317005 5 µg
RBG10317025 25 µg
RBG10317100 100 μg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2–6 bisphosphate (F-2–6-BP) to fructose-6-phosphate and inorganic phosphate. F-2–6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2–6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Recombinant Human TIGAR expressed in E. coli is a 29.9 kDa protein containing 269 amino acid residues.

Amino Acid Sequence

ARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGIFLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSELRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSNCLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLPATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETR

Source

E. coli

Purity

95%

Biological Activity

Data not available.

Endotoxin

Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5–1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at –20°C to –80°C.

Storage/Expiration

 –20°C

Summary

Research topic

Apoptosis, Oncology

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