Tumor Necrosis Factor-a Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17339.44 Dalton. The TNF-alpha is purified by standard chromatographic techniques.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Ser-Ser.
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05 ng/ml, corresponding to a Specific Activity of 5×107 IU/mg.
The protein was lyophilized with no additives.
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution TNF-a should be stored at 4°C between 2–7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Sterile filtered white lyophilized (freeze-dried) powder.