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Distributed product

Total Estriol ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA
  • Other names:E3
  • Species:Human
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Cat. No. Size Price

New DKO019 96 wells (1 kit)
PubMed Product Details
Technical Data


Competitive ELISA


Serum, Plasma

Sample Requirements

20 µl/well


Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

2 - 200 ng/ml

Limit of Detection

1 ng/ml

Intra-assay (Within-Run)

CV ≤ 9.9%

Inter-assay (Run-to-Run)

CV ≤ 10.3%

Spiking Recovery


Dilution Linearity



The kits are CE-IVD certified and intended for professional use.



European Union: for in vitro diagnostic use

Rest of the world: for research use only!

The total assay time is less than 1.5 hours

Quantitative determination of total estriol concentration in human serum and plasma

Assay format is 96 wells

Quality Controls (A and B) are ready to use

Standards are ready to use

For routine analysis

Research topic

Autoimmunity, Reproduction, Steroid hormones


Estriol is one of the three main estrogens produced by the human body. It is only produced in significant amounts during pregnancy as it is made by the fetus. During pregnancy the production of estriol depends on an intact maternal- placental-fetal unit. Fetal-placental production of estriol leads to a progressive rise in maternal circulating levels reaching a late-gestational peak several orders of magnitude greater than non- pregnant levels. In the maternal circulation, estriol undergoes rapid conjugation in the liver followed by urinary excretion with a half-life of ~20 minutes. Since normal estriol production depends on an intact maternal-placental-fetal circulation and functional fetal metabolism, maternal estriol levels have been used to monitor fetal status during pregnancy, particularly during the third trimester. DHEA is produced by the adrenal cortex of the fetus, this is converted to estriol by the placenta. If levels are abnormally low in a pregnant woman, this may indicate a problem with the development in the child. Levels of estriol in non-pregnant women do not change much after menopause, and levels are not significantly different from levels in men.

Summary References (1)

References to Estriol

  • Bashore RA, Westlake JR. Plasma unconjugated estriol values in high-risk pregnancy. Am J Obstet Gynecol. 1977 Jun 15;128 (4):371-80
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