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Manufactured by BioVendor

Total Prorenin/Renin Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Species:Human
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Cat. No. Size Price


New RD191167200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Urine, Amniotic fluid

Sample Requirements

50 µl/well

Storage/Expiration

Store the complete kit at 2 8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Limit of Detection

6 pg/ml

Intra-assay (Within-Run)

n=8 CV= 5%

Inter-assay (Run-to-Run)

n=6 CV= 6%

Spiking Recovery

100.4%

Dilutation Linearity

96.2%

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3.5 hours
  • The kit measures prorenin and renin in serum, plasma (EDTA, citrate, heparin), urine and amniotic fluid
  • Assay format is 96 wells
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Blood pressure regulation and NO metabolism, Diabetology - Other Relevant Products , Renal disease

Summary

Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects.

References to Summary

References to Prorenin

  • Batenburg WW, Danser AH. (Pro)renin and its receptors: pathophysiological implications. Clin Sci (Lond). 2012 Aug 01;123 (3):121-33
  • Danser AH. Prorenin: back into the arena. Hypertension. 2006 May;47 (5):824-6
  • Danser AH, Derkx FH, Schalekamp MA, Hense HW, Riegger GA, Schunkert H. Determinants of interindividual variation of renin and prorenin concentrations: evidence for a sexual dimorphism of (pro)renin levels in humans. J Hypertens. 1998 Jun;16 (6):853-62
  • Hsueh WA, Baxter JD. Human prorenin. Hypertension. 1991 Apr;17 (4):469-77
  • Ichihara A, Sakoda M, Kurauchi-Mito A, Kaneshiro Y, Itoh H. Renin, prorenin and the kidney: a new chapter in an old saga. J Nephrol. 2009 May-Jun;22 (3):306-11
  • Jan Danser AH, Batenburg WW, van Esch JH. Prorenin and the (pro)renin receptor--an update. Nephrol Dial Transplant. 2007 May;22 (5):1288-92
  • Luetscher JA, Kraemer FB, Wilson DM, Schwartz HC, Bryer-Ash M. Increased plasma inactive renin in diabetes mellitus. A marker of microvascular complications. N Engl J Med. 1985 May 30;312 (22):1412-7
  • Mikami Y, Narita T, Takai Y, Baba K, Seki H, Matsuoka K. The progression of serum prorenin concentration during pregnancy. J Hypertens . 2014;3 (4)
  • Paul M, Poyan Mehr A, Kreutz R. Physiology of local renin-angiotensin systems. Physiol Rev. 2006 Jul;86 (3):747-803
  • Pringle KG, Wang Y, Lumbers ER. The synthesis, secretion and uptake of prorenin in human amnion. Physiol Rep. 2015 Apr;3 (4)
  • Schalekamp MA, Derkx FH, Deinum J, Danser AJ. Newly developed renin and prorenin assays and the clinical evaluation of renin inhibitors. J Hypertens. 2008 May;26 (5):928-37
  • Schmieder RE. The potential role of prorenin in diabetic nephropathy. J Hypertens. 2007 Jul;25 (7):1323-6
  • Shi P, Grobe JL, Desland FA, Zhou G, Shen XZ, Shan Z, Liu M, Raizada MK, Sumners C. Direct pro-inflammatory effects of prorenin on microglia. PLoS One. 2014;9 (10):e92937
  • Stankovic AR, Fisher ND, Hollenberg NK. Prorenin and angiotensin-dependent renal vasoconstriction in type 1 and type 2 diabetes. J Am Soc Nephrol. 2006 Dec;17 (12):3293-9
  • Toffelmire EB, Slater K, Corvol P, Menard J, Schambelan M. Response of plasma prorenin and active renin to chronic and acute alterations of renin secretion in normal humans. Studies using a direct immunoradiometric assay. J Clin Invest. 1989 Feb;83 (2):679-87
  • Yokota H, Mori F, Kai K, Nagaoka T, Izumi N, Takahashi A, Hikichi T, Yoshida A, Suzuki F, Ishida Y. Serum prorenin levels and diabetic retinopathy in type 2 diabetes: new method to measure serum level of prorenin using antibody activating direct kinetic assay. Br J Ophthalmol. 2005 Jul;89 (7):871-3
  • Yokota H, Nagaoka T, Tani T, Takahashi A, Sato E, Kato Y, Yoshida A. Higher levels of prorenin predict development of diabetic retinopathy in patients with type 2 diabetes. J Renin Angiotensin Aldosteron. 2011 Sep;12 (3):290-4
  • Yoshida G, Kawasaki M, Murata I, Hayakawa Y, Aoyama T, Miyazaki N, Yamada Y, Nishigaki K, Arai Y, Suzuki F, Minatoguchi S. Higher plasma prorenin concentration plays a role in the development of coronary artery disease. Biomark Res. 2015;3:18
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