Immunocapture enzyme activity assay
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Limit of Detection
n = 8; CV = 1.6%
n = 4; CV = 7.0%
- bovine Non-detectable
- cat Non-detectable
- goat Non-detectable
- hamster Non-detectable
- horse Non-detectable
- dog Yes
- mouse Non-detectable
- pig Non-detectable
- rat Non-detectable
- sheep Non-detectable
- chicken Not tested
- monkey Yes
- rabbit Yes
- human Yes
- European Union: for in vitro diagnostic use
- Rest of the world: for research use only!
- The total assay time is less than 4 hours
- The kit measures TRAP 5 proteins exhibiting enzyme activity in serum and EDTA plasma
- Assay format is 96 wells
- Quality Controls are recombinant TRAP 5 protein based. No animal sera are used
- Calibrators are recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Bone and cartilage metabolism
TRAP 5 (serum band 5 tartrate-resistant acid phosphatase, TRACP 5; EC 220.127.116.11) is a glycoprotein of 35–37 kDa. TRAP 5 belongs to the most abundant enzymes in osteoclasts. It is expressed in certain differentiated cells of the mononuclear phagocyte system, particularly osteoclasts and alveolar macrophages, where it takes an active part in bone resorption process. High blood levels of TRAP 5 are usually associated with active bone remodelling. Increased serum levels are observed during normal bone growth among healthy children. Elevated serum TRAP levels have been detected in diseases characterized by increased bone resorption; Paget’s disease of the bone, hemodialysis, primary hyperparathyroidism, metastatic malignancies involving bone resorption, multiple myeloma and bilaterally ovariectomized women. Post-menopausal women have higher levels of serum than post-menopausal women on estrogen replacement therapy. Therefore specific determination of TRAP 5 activity can be essential for clinical assessment of bone metabolism.