Total 162 AA. MW: 18.0 kDa (calculated). UniProtKB acc. no. O43508 (Ser94-His249). C-terminal His-tag (5 extra AA). Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
Purity as determined by densitometric image analysis: > 95 %
14 % SDS-PAGE separation of Human TWEAK:
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. Reduced and boiled sample, 2.5 μg/lane
3. Non-reduced and non-boiled sample, 2.5 μg/lane
< 1.0 EU/μg
Filtered (0.4 μm) andand frozen from 0.30 – 0.60 mg/mL in 20 mM Tris buffer, 900 mM NaCl, 20% vol/vol glycerol, pH 7.5.
Defrost at ambient temperature.Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
Western blotting, ELISA
On ice. Upon receipt, store the product at the temperature recommended below.
Store the protein at –80 °C. Frozed protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Thawed protein can be stored at 4 °C for a week.
This product is intended for research use only.