Native protein isolated from canine urine, 589 AA, MW: 64,483 kDa (calculated without glycosylation). Protein identity confirmed by LC-MS/MS (NCBI no. gi|50950249).
Amino Acid Sequence
SDS-PAGE analysis of canine uromodulin native protein, 12% gel stained with Coomassie Brillant Blue G250
- M.W. marker – 14, 21, 31, 45, 66, 97 kDa
- reduced and heated sample, 2.5ug/lane
- non-reduced and non-heated sample, 2.5μg/lane
< 1.0 EU/ug
Filtered (0,4 μm) and lyophilized in 0.1 mg/mL in deionized water.
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Western blotting, ELISA, Cell culture and/or animal studies, Immunological methods
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
This product is intended for research use only.