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Using Retriever

The solution for antigen unmasking

Our Unique Retriever solves the problem associated with staining formalin fixed tissues. It is an affordable solution to all known major problems with immunohistochemistry on paraffin sections. Ease of use combined with high reproducibility of the results will help you achieve the best quality immunostaining. The Retriever is a bench-top model for thermally processing slides of formalin-fixed, paraffin embedded tissues prior to immunostaining.​​​

The model has been designed to ensure identical processing of all the samples during a processing cycle, as well as the identical processing of the samples in individual sessions. The Retriever preserves processed tissues.

How does the Retriever work?

It looks like a pressure cooker, and basically it is an advanced pressure cooker. People ask sometimes what temperature and pressure are used (to compare it with some other pressure cooker). However, this information will not be helpful, because the key factor is not what temperature and pressure it reaches, but, rather, how the heating profile is dictated by a microchip controlling the process, and how the proper heating profile is tested during production for every individual unit of the Retriever. Sensors control the heating profile controlling the temperature and pressure to be reached at a certain pace and over a certain time period. When the required temperature is reached, it will be held for several minutes. After that the slides are cooled for 2 hours. Specially designed thermal walls of the unit control the speed of cooling of the inner chamber and slides. This is an important stage of the process as well: it gives proteins a chance to re-fold some domains after the processing.

With Retriever you can

  • Run antigen unmasking in up to 6 different buffers in one cycle. This is very important when you are trying to find the most appropriate buffer for epitope recovery or need to process the same tissue in different buffers for different antibodies.
  • Use a minimal quantity of processing buffer (thus always using a fresh one without high cost, and not jeopardising the identical processing and thus identical staining on tissues from different cycles).
  • Recover the epitope of interest for a strong staining while preserving the tissue and cell morphology (quite important in diagnostic pathology).
  • Get identical results every time even in experiments that are performed months apart. This is especially critical for retrospective research that involves IHC on large collections of tissues that may not be all available at once. Using Retriever gives you the confidence that all stainings will be performed on identically retrieved sections
  • Just do it. Load the sections, pour the buffer, close the lid and push the button. No training, no “tricks of the trade”, no waiting when the diagnostic pathology has time to do it for you.
  • Use either standard Citrate of EDTA buffers, or our buffers (new Universal set of buffers arrives this fall).
  • No worries that the machine will stop working. This only happened twice in the history of Retriever, and both times it was the result of improper usage. In any case, you have a full year of replacement guarantee.

Who would benefit from using our Retriever?

  • Investigative Pathology, where the high quality of staining (a picture may be published!) is required.
  • Any lab that is short on technical personnel: any student or post-doc can process slides for the staining with little time requirements.
  • Small routine pathology labs, where a limited number of slides should be processed daily.
  • Anyone who uses highly valuable samples, such as tissue arrays or unique tissue samples.
  • Simplicity and reliability of the unit ensures the safety of your sample, and a high quality antigen unmasking.

Files

Quick Start Guide
Retrivere Scheme

Product Size Price
1.5mm

Type: Puncher Tip

TAT06-1.5 1 ea
 
1.5mm (10 x 15 ; 150 wells)

Type: Mold Kit

TAM01-1.5 1 ea
 
1.5mm (9 x 10 ; 90 wells)

Type: Premade Recipient Block

TAB06-1.5 1 ea
 
1mm

Type: Puncher Tip

TAT06-1 1 ea
 
1mm (10 x 12 ; 120 wells)

Type: Premade Recipient Block

TAB06-1 1 ea
 
1mm (10 x 17 ; 170 wells)

Type: Mold Kit

TAM01-1 1 ea
 
2mm

Type: Puncher Tip

TAT06-2 1 ea
 
2mm (6 x 10 ; 60 wells)

Type: Premade Recipient Block

TAB06-2 1 ea
 
2mm (7x 10 ; 70 wells)

Type: Mold Kit

TAM01-2 1 ea
 
3mm

Type: Puncher Tip

TAT06-3 1 ea
 
3mm (5 x 6 ; 30 wells)

Type: Premade Recipient Block

TAB06-3 1 ea
 
3mm (5 x 8 ; 40 wells)

Type: Mold Kit

TAB01-3 1 ea
 
5mm

Type: Puncher Tip

TAT06-5 1 ea
 
5mm (3 x 5 ; 15 wells)

Type: Mold Kit

TAB-01-5 1 ea
 
5mm (4 x 5 ; 20 wells)

Type: Premade Recipient Block

TAB06-5 1 ea
 
Antibody Diluent For Frozen Sections ready-to-use

Type: IHC Buffers

AP0475-050 50 ml
 
Antibody Diluent For Frozen Sections ready-to-use

Type: IHC Buffers

AP0475-125 125 ml
 
Antibody Diluent For Frozen Sections ready-to-use

Type: IHC Buffers

AP0475-500 500 ml
 
Antibody Diluent ready-to-use

Type: IHC Buffers

AP0742-050 50 ml
 
Antibody Diluent ready-to-use

Type: IHC Buffers

AP0472-125 125 ml
 
Antibody Diluent ready-to-use

Type: IHC Buffers

AP0472-500 500 ml
 
Antibody Diluent W ready-to-use

Type: Western Blot Buffers

AP0501-050 50 ml
 
Antibody Diluent W ready-to-use

Type: Western Blot Buffers

AP0501-125 125 ml
 
Antibody Diluent W ready-to-use

Type: Western Blot Buffers

AP0501-500 500 ml
 
Base mold

Type: Mold Kit

TABM06 1 ea
 
Basket

Type: Accessories

R21008 1 each
 
Basket Lid for Chambers

Type: Accessories

R21009 1 each
 
Coating Buffer 7.4

Type: ELISA

AP0511-500 500 ml
 
Coating Buffer 9.6

Type: ELISA

AP0510-500 500 ml
 
Cord Set EU

Type: Accessories

R21005 1 each
 
Embedding cassette

Type: Mold Kit

TAC06 500/PK
 
Full set with 5 Tips and 5 sized blocks

Type: Tissue Microarrayer Set

TAT06 1 set
 
Green Silicone Sealing Gasket

Type: Accessories

R21007 1 each
 
Guide for 1mm recipient block

Type: Puncher Tip

TAG06 1 set
 
Peroxidase Conjugate Diluent ready-to-use

Type: Western Blot Buffers

AP0473-050W 50 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: Western Blot Buffers

AP0473-125W 125 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: Western Blot Buffers

AP0473-500W 500 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: ELISA

AP0473-050E 50 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: ELISA

AP0473-125E 125 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: ELISA

AP0473-500E 500 m
 
Peroxidase Conjugate Diluent ready-to-use

Type: IHC Buffers

AP0473-050 50 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: IHC Buffers

AP0473-125 125 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: IHC Buffers

AP0473-500 500 ml
 
Plate Block E

Type: ELISA

AP0513-500 500 ml
 
Plate Block, No Components of Animal Origin

Type: ELISA

AP0512-125 125 ml
 
Retriever Unit

Type: Retriever Unit

R2100-EU 1 each
 
Sample & Antibody Diluent E ready-to-use

Type: ELISA

AP0514-050 50 ml
 
Sample & Antibody Diluent E ready-to-use

Type: ELISA

AP0514-125 125 ml
 
Sample & Antibody Diluent E ready-to-use

Type: ELISA

AP0514-500 500 ml
 
Section Block ready-to-use

Type: IHC Buffers

AP0471-050 50 ml
 
Section Block ready-to-use

Type: IHC Buffers

AP0471-125 125 ml
 
Single set for 1 mm puncher with a guide

Type: Tissue Microarrayer Set

TAT06T1 1 set
 
Single set for 1.5mm puncher

Type: Tissue Microarrayer Set

TAT06T1.5 1 set
 
Single set for 2mm puncher

Type: Tissue Microarrayer Set

TAT06T2 1 set
 
Single set for 3mm puncher

Type: Tissue Microarrayer Set

TAT06T3 1 set
 
Single set for 5mm puncher

Type: Tissue Microarrayer Set

TAT06T5 1 set
 
Slide Chamber

Type: Accessories

R21001 Set of 3
 
Stripping Buffer ready-to-use W

Type: Western Blot Buffers

AP0503-050 50 ml
 
Stripping Buffer ready-to-use W

Type: Western Blot Buffers

AP0503-125 125 ml
 
Stripping Buffer ready-to-use W

Type: Western Blot Buffers

AP0503-500 500 ml
 
Universal Buffer

Type: Universal Buffers

AP0530-125 125 ml, 10x
 
Universal Buffer

Type: Universal Buffers

AP0530-500 500 ml, 10x
 
Washing Buffer E PBS-Based 10x

Type: ELISA

AP0515-500 500 ml
 
Washing Buffer W Tris-Based 10x

Type: Western Blot Buffers

AP0502-500 500 ml
 
Western Block, BSA-Free ready-to-use

Type: Western Blot Buffers

AP0500-125 125 ml
 
Western Block, BSA-Free ready-to-use

Type: Western Blot Buffers

AP0500-500 500 ml