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Using Retriever

The solution for antigen unmasking

Our Unique Retriever solves the problem associated with staining formalin fixed tissues. It is an affordable solution to all known major problems with immunohistochemistry on paraffin sections. Ease of use combined with high reproducibility of the results will help you achieve the best quality immunostaining. The Retriever is a bench-top model for thermally processing slides of formalin-fixed, paraffin embedded tissues prior to immunostaining.​​​

The model has been designed to ensure identical processing of all the samples during a processing cycle, as well as the identical processing of the samples in individual sessions. The Retriever preserves processed tissues.

How does the Retriever work?

It looks like a pressure cooker, and basically it is an advanced pressure cooker. People ask sometimes what temperature and pressure are used (to compare it with some other pressure cooker). However, this information will not be helpful, because the key factor is not what temperature and pressure it reaches, but, rather, how the heating profile is dictated by a microchip controlling the process, and how the proper heating profile is tested during production for every individual unit of the Retriever. Sensors control the heating profile controlling the temperature and pressure to be reached at a certain pace and over a certain time period. When the required temperature is reached, it will be held for several minutes. After that the slides are cooled for 2 hours. Specially designed thermal walls of the unit control the speed of cooling of the inner chamber and slides. This is an important stage of the process as well: it gives proteins a chance to re-fold some domains after the processing.

With Retriever you can

  • Run antigen unmasking in up to 6 different buffers in one cycle. This is very important when you are trying to find the most appropriate buffer for epitope recovery or need to process the same tissue in different buffers for different antibodies.
  • Use a minimal quantity of processing buffer (thus always using a fresh one without high cost, and not jeopardising the identical processing and thus identical staining on tissues from different cycles).
  • Recover the epitope of interest for a strong staining while preserving the tissue and cell morphology (quite important in diagnostic pathology).
  • Get identical results every time even in experiments that are performed months apart. This is especially critical for retrospective research that involves IHC on large collections of tissues that may not be all available at once. Using Retriever gives you the confidence that all stainings will be performed on identically retrieved sections
  • Just do it. Load the sections, pour the buffer, close the lid and push the button. No training, no “tricks of the trade”, no waiting when the diagnostic pathology has time to do it for you.
  • Use either standard Citrate of EDTA buffers, or our buffers (new Universal set of buffers arrives this fall).
  • No worries that the machine will stop working. This only happened twice in the history of Retriever, and both times it was the result of improper usage. In any case, you have a full year of replacement guarantee.

Who would benefit from using our Retriever?

  • Investigative Pathology, where the high quality of staining (a picture may be published!) is required.
  • Any lab that is short on technical personnel: any student or post-doc can process slides for the staining with little time requirements.
  • Small routine pathology labs, where a limited number of slides should be processed daily.
  • Anyone who uses highly valuable samples, such as tissue arrays or unique tissue samples.
  • Simplicity and reliability of the unit ensures the safety of your sample, and a high quality antigen unmasking.

Files

Quick Start Guide
Retrivere Scheme