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Manufactured by BioVendor

VEGF-A Mouse ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Vascular endothelial growth factor A, VPF
  • Species:Mouse
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Cat. No. Size Price


RAF137R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Cell culture supernatant

Sample Requirements

50 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

62.5–4000 pg/ml

Limit of Detection

20 pg/ml

Intra-assay (Within-Run)

CV = 3.8%

Inter-assay (Run-to-Run)

CV = 6.4%

Summary

Research topic

Cytokines and chemokines and related molecules, Oncology, Animal studies

Summary

VEGF is characterized by its highly specific mitogenic activity in endothelial cells and its angiogenic effect observed in vitro and in vivo. The VEGF family consists of four isoforms (A-D). VEGF-A is considered the most important of these with respect to tumor angiogenesis. and the splice variant isoforms VEGF121 and VEGF165 were found to be secreted by a wide spectrum of cell types, including smooth muscle cells, fibroblasts, epithelial cells, keratinocytes, macrophages, cardiac myocytes and various tumor cells. Inhibition of VEGF with a specific monoclonal antibody can suppress tumor growth in vivo.

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