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Manufactured by BioVendor

VILIP-1 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Visinin-Like Protein 1, Hippocalcin-like protein 3, HLP3, VILIP, VLP-1, VSNL1, VISL1
  • Species:Human
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Cat. No. Size Price


RD191119200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Cerebrospinal fluid, Tissue extract, Plasma

Sample Requirements

100 ul/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.1–5 ng/ml

Limit of Detection

0.027 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 5.3%

Inter-assay (Run-to-Run)

n = 5; CV = 3.2%

Spiking Recovery

104,20%

Dilutation Linearity

94,30%

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3.5 hours
  • The kit measures human VILIP-1 in serum, plasma, CSF and tissue extracts
  • Assay format is 96 wells
  • Quality Controls are human serum based. No animal sera are used
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Neural tissue markers, Oncology

Summary

Visinin like protein 1 (VILIP-1, VLP-1 or VSNL-1) is a cytoplasmic protein of low molecular weight (approximately 22 kDa) consisting of 191 amino acid residues. It belongs to the visinin/recoverin subfamily of neuronal calcium sensor proteins involved in calcium-dependent signal transduction mechanisms in neurons. It is found primarily in the brain, in nerve cells, but it also has a peripheral distribution in liver, lung, kidney, spleen, pancreas and colon. When localized at the membrane, it modulates various cellular signal transduction pathways, including cyclic adenosine monophosphate (cAMP)- and cyclic guanosine monophosphate (cGMP)-signaling in neural cells, human embryonic kidney cells, the pancreatic β cell line MIN6, and various skin tumor cell lines. It contains four internal repeats of 36–38 amino-acids, each containing a potential EF-hand domain. Two of the four EF-hand Ca2+-binding motifs of VILIP-1 are able to bind either Ca2+ or Mg2+in a non-cooperative manner. Binding of Ca2+ leads to specific conformational changes in the protein and this may regulate the interaction of VILIP with intracellular target molecules. VILIP-1 has been identified as a potential biomarker for brain injury and several neurodegenerative diseases. VILIP-1-expressing cells appear to be vulnerable to neurotoxic insults. As a result, the protein is released into the cerebrospinal fluid (CSF), and can be used as a biomarker for stroke and Alzheimer’s di­sease. The intracellular protein was detected in cerebrospinal fluid (CSF) of a rat model of stroke and in plasma of patients after stroke. VILIP-1 was detected in 44% of subjects with stroke, in samples taken 24 hours after onset of stroke, and in 8% of controls with no stroke. In Alzheimer’s di­sease, CSF levels of VILIP-1 were significantly higher than in healthy individuals. Post mortem studies in the hippocampus of schizophrenia patients revealed increased expression of VILIP-1 in interneurons, while its expression in pyramidal neurons was downregulated. Expressions of VILIP-1 were also found in different types of cancer and in pancreatic α- and β-cells, being involved in the regulation of insulin secretion and insulin gene expression.

Product References (3)

References

  • Babić Leko M, Willumsen N, Nikolac Perković M, Klepac N, Borovečki F, Hof PR, Sonicki Z, Pivac N, de Silva R, Šimić G. Association of MAPT haplotype-taggingpolymorphisms with cerebrospinal fluid biomarkers of Alzheimer's disease: Apreliminary study in a Croatian cohort. Brain Behav. 2018 Nov;8(11):e01128. doi: 10.1002/brb3.1128. Epub 2018 Oct 17. PubMed PMID: 30329219; PubMed Central PMCID:PMC6236251. See more on PubMed
  • Hasegawa S, Matsushige T, Inoue H, Takahara M, Kajimoto M, Momonaka H, Oka M, Isumi H, Emi S, Hayashi M, Ichiyama T. Serum and cerebrospinal fluid levels ofvisinin-like protein-1 in acute encephalopathy with biphasic seizures and latereduced diffusion. Brain Dev. 2014 Aug;36(7):608-12. doi:10.1016/j.braindev.2013.08.010. Epub 2013 Sep 26. PubMed PMID: 24075506. See more on PubMed
  • Jensen CS, Portelius E, Høgh P, Wermuth L, Blennow K, Zetterberg H,Hasselbalch SG, Simonsen AH. Effect of physical exercise on markers of neuronaldysfunction in cerebrospinal fluid in patients with Alzheimer's disease.Alzheimers Dement (N Y). 2017 Apr 17;3(2):284-290. doi:10.1016/j.trci.2017.03.007. eCollection 2017 Jun. PubMed PMID: 29067334; PubMedCentral PMCID: PMC5651430. See more on PubMed
Summary References (13)

References to VILIP-1

  • Brackmann M, Schuchmann S, Anand R, Braunewell KH. Neuronal Ca2+ sensor protein VILIP-1 affects cGMP signalling of guanylyl cyclase B by regulating clathrin-dependent receptor recycling in hippocampal neurons. J Cell Sci. 2005 Jun 1;118 (Pt 11):2495-505
  • Braunewell KH. VILIP-1. UCSD-Nature Molecule Pages. 2009;
  • Braunewell KH, Klein-Szanto AJ. Visinin-like proteins (VSNLs): interaction partners and emerging functions in signal transduction of a subfamily of neuronal Ca2+ -sensor proteins. Cell Tissue Res. 2009 Feb;335 (2):301-16
  • Burgoyne RD. The neuronal calcium- sensor proteins. Biochimica et Biophysica Acta. 2004;
  • Chen KC, Wang LK, Chang LS. Regulatory elements and functional implication for the formation of dimeric visinin-like protein-1. J Pept Sci. 2009 Feb;15 (2):89-94
  • Dai FF, Zhang Y, Kang Y, Wang Q, Gaisano HY, Braunewell KH, Chan CB, Wheeler MB. The neuronal Ca2+ sensor protein visinin-like protein-1 is expressed in pancreatic islets and regulates insulin secretion. J Biol Chem. 2006 Aug 4;281 (31):21942-53
  • Fu J, Fong K, Bellacosa A, Ross E, Apostolou S, Bassi DE, Jin F, Zhang J, Cairns P, Ibanez de Caceres I, Braunewell KH, Klein-Szanto AJ. VILIP-1 downregulation in non-small cell lung carcinomas: mechanisms and prediction of survival. PLoS One. 2008;3 (2):e1698
  • Gierke P, Zhao C, Bernstein HG, Noack C, Anand R, Heinemann U, Braunewell KH. Implication of neuronal Ca2+ -sensor protein VILIP-1 in the glutamate hypothesis of schizophrenia. Neurobiol Dis. 2008 Oct;32 (1):162-75
  • Laterza OF, Modur VR, Crimmins DL, Olander JV, Landt Y, Lee JM, Ladenson JH. Identification of novel brain biomarkers. Clin Chem. 2006 Sep;52 (9):1713-21
  • Lee JM, Blennow K, Andreasen N, Laterza O, Modur V, Olander J, Gao F, Ohlendorf M, Ladenson JH. The brain injury biomarker VLP-1 is increased in the cerebrospinal fluid of Alzheimer disease patients. Clin Chem. 2008 Oct;54 (10):1617-23
  • Lin L, Jeanclos EM, Treuil M, Braunewell KH, Gundelfinger ED, Anand R. The calcium sensor protein visinin-like protein-1 modulates the surface expression and agonist sensitivity of the alpha 4beta 2 nicotinic acetylcholine receptor. J Biol Chem. 2002 Nov 1;277 (44):41872-8
  • Schnurra I, Bernstein HG, Riederer P, Braunewell KH. The neuronal calcium sensor protein VILIP-1 is associated with amyloid plaques and extracellular tangles in Alzheimer's disease and promotes cell death and tau phosphorylation in vitro: a link between calcium sensors and Alzheimer's disease?. Neurobiol Dis. 2001 Oct;8 (5):900-9
  • Verbeek MM, Olde Rikkert MG. Cerebrospinal fluid biomarkers in the evaluation of Alzheimer disease. Clin Chem. 2008 Oct;54 (10):1589-91
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