Recombinant Human Vitronectin, expressed in HEK293 cells. Calculated molecular weight of 52.2 kDa.
Amino Acid Sequence
Recombinant Human Vitronectin promotes attachment of hESC and iPSC in serum-free, feeder conditions at 5 μg/ml.
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at-20°C to –80°C.
Store lyophilized protein at –20°C to –80°C. Lyophilized protein remains stable until the expiry date when stored at –20°C to –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at 2°C to 8°C for up to one week, or –20°C to –80°C for extended storage up to 3 months.