Western Blot Buffers
W is for Western
Based on the same technology as our buffers for IHC applications, we make specially adjusted buffers for improving sensitivity and decreasing background in Western-blot applications.
Western Block (# RAP0500) ready-to-use
Specialised solution for blocking free binding sites in Western blots on PVDF, or various types of nitrocellulose membrane. It does not contain large proteins, including serum proteins, BSA, cows milk proteins; low-molecular weight blocking components of this blocking solution do not cause non-specific binding of antibodies to blocking ingredients, while effectively preventing non-specific interactions of test antibody with the membrane and transferred proteins on the blot. They also do not prevent (sterically) recognition of the target proteins on the blot due to the size. The buffer is an excellent alternative to self-made skim milk solutions as it is more effective and improves the signal to noise ratio. Contains ProClin® 300 as preservative and has a shelf life of over 12 months at 2–8°C.
Antibody Diluent W (# RAP0501) ready-to-use
Specifically designed for Western blot and other solid phase immunoassays, such as immune-PCR, dot blot, protein arrays, this buffer prevents non-specific binding of antibody to the solid phase and proteins. As a result, using Antibody diluent may dramatically improve the signal/noise ratio and increase the sensitivity of your assay. Contains ProClin® 300 as preservative and has a shelf life of over 12 months at 2–8°C.
Stripping Buffer W (#RAP0503) ready-to-use
Specially designed for re-probing Western blots, detected with chemiluminiscent and other methods with non-precipitating substrate (i.e. immunofluorescent detection).This formulation effectively and gently removes primary and secondary antibodies from the blot, thus allowing you to re-probe it again with a new specific antibody.
for standardising you procedures, you may also be interested in:
Washing Buffer W Tris-Based (# RAP0502) 10× stock
A concentrated stock buffer to rinse and wash the western blot between the procedures without removing blocking and other agents preventing the non-specific reactivity of immunochemicals during the detection procedure. Contains ProClin® 300 as preservative and has shelf life of over 12 months at 2–8°C.