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Why choose the Retriever?

​​​​​​“…the key word about Retriever is reproducibility. Due to fully automatic processing the stainings are always the same, whether they are done within a few hours or a few months apart…”

Not only for diagnostics, but also for research (and perhaps especially for research pathology), standardization of staining is the key word.

Retriever instrumentation that enables epitope retrieval by the push of a button, has been created precisely to assure standard staining. In contrast to simple “pressure-cookers”, Retriever allows proper recovery of epitopes on formalin-fixed (routine pathology sections) while preserving tissue morphology, shapes of cells, shape of the cell's nucleus, and always giving you the same quality and degree of fixed tissue recovery.

Since its launch in 2006 it has been further refined, and today may well be the most advanced and recommended epitope recovery unit.

Technology

Retriever is more than a “pressure cooker”. A chip on board the machine controls the profile of heating, pressure and the length of the cycle optimal for most of the routine formalin-fixed, paraffin embedded tissues. Routine pathology tissues may differ quite substantially by the degree of fixation, temperature during embedding, etc. The Retriever is the result of over 10 years of work on the optimization of the parameters for the best method of unmasking of the antigens in a wide variety of tissues.

Advantages

First, Retriever requires no special training. Even a new trainee in the lab can use it. Second, it allows simultaneously using several different buffers (often some of your antibodies require citrate buffer, other – acid citrate or – EDTA) within one cycle, in one machine. Third, it gives high reproducibility of results, which allows comparsions of stainings performed at two widely-spaced time points. Fourth, it is very reliable. Since first production only 2 machines have had to be replaced. So you can expect many years of uninterrupted work with Retriever.

6692

6691

Proper epitope recovery may be critical for your conclusions on protein expression. The pictures show staining for E-cadherin on human small intestine tissue without processing in Retriever (top) and with the processing on Retriever (bottom).

Product Size Price
1.5mm

Type: Puncher Tip

TAT06-1.5 1 ea
 
1.5mm (10 x 15 ; 150 wells)

Type: Mold Kit

TAM01-1.5 1 ea
 
1.5mm (9 x 10 ; 90 wells)

Type: Premade Recipient Block

TAB06-1.5 1 ea
 
1mm

Type: Puncher Tip

TAT06-1 1 ea
 
1mm (10 x 12 ; 120 wells)

Type: Premade Recipient Block

TAB06-1 1 ea
 
1mm (10 x 17 ; 170 wells)

Type: Mold Kit

TAM01-1 1 ea
 
2mm

Type: Puncher Tip

TAT06-2 1 ea
 
2mm (6 x 10 ; 60 wells)

Type: Premade Recipient Block

TAB06-2 1 ea
 
2mm (7x 10 ; 70 wells)

Type: Mold Kit

TAM01-2 1 ea
 
3mm

Type: Puncher Tip

TAT06-3 1 ea
 
3mm (5 x 6 ; 30 wells)

Type: Premade Recipient Block

TAB06-3 1 ea
 
3mm (5 x 8 ; 40 wells)

Type: Mold Kit

TAB01-3 1 ea
 
5mm

Type: Puncher Tip

TAT06-5 1 ea
 
5mm (3 x 5 ; 15 wells)

Type: Mold Kit

TAB-01-5 1 ea
 
5mm (4 x 5 ; 20 wells)

Type: Premade Recipient Block

TAB06-5 1 ea
 
Antibody Diluent For Frozen Sections ready-to-use

Type: IHC Buffers

AP0475-050 50 ml
 
Antibody Diluent For Frozen Sections ready-to-use

Type: IHC Buffers

AP0475-125 125 ml
 
Antibody Diluent For Frozen Sections ready-to-use

Type: IHC Buffers

AP0475-500 500 ml
 
Antibody Diluent ready-to-use

Type: IHC Buffers

AP0742-050 50 ml
 
Antibody Diluent ready-to-use

Type: IHC Buffers

AP0472-125 125 ml
 
Antibody Diluent ready-to-use

Type: IHC Buffers

AP0472-500 500 ml
 
Antibody Diluent W ready-to-use

Type: Western Blot Buffers

AP0501-050 50 ml
 
Antibody Diluent W ready-to-use

Type: Western Blot Buffers

AP0501-125 125 ml
 
Antibody Diluent W ready-to-use

Type: Western Blot Buffers

AP0501-500 500 ml
 
Base mold

Type: Mold Kit

TABM06 1 ea
 
Basket

Type: Accessories

R21008 1 each
 
Basket Lid for Chambers

Type: Accessories

R21009 1 each
 
Coating Buffer 7.4

Type: ELISA

AP0511-500 500 ml
 
Coating Buffer 9.6

Type: ELISA

AP0510-500 500 ml
 
Cord Set EU

Type: Accessories

R21005 1 each
 
Embedding cassette

Type: Mold Kit

TAC06 500/PK
 
Full set with 5 Tips and 5 sized blocks

Type: Tissue Microarrayer Set

TAT06 1 set
 
Green Silicone Sealing Gasket

Type: Accessories

R21007 1 each
 
Guide for 1mm recipient block

Type: Puncher Tip

TAG06 1 set
 
Peroxidase Conjugate Diluent ready-to-use

Type: Western Blot Buffers

AP0473-050W 50 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: Western Blot Buffers

AP0473-125W 125 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: Western Blot Buffers

AP0473-500W 500 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: ELISA

AP0473-050E 50 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: ELISA

AP0473-125E 125 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: ELISA

AP0473-500E 500 m
 
Peroxidase Conjugate Diluent ready-to-use

Type: IHC Buffers

AP0473-050 50 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: IHC Buffers

AP0473-125 125 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: IHC Buffers

AP0473-500 500 ml
 
Plate Block E

Type: ELISA

AP0513-500 500 ml
 
Plate Block, No Components of Animal Origin

Type: ELISA

AP0512-125 125 ml
 
Retriever Unit

Type: Retriever Unit

R2100-EU 1 each
 
Sample & Antibody Diluent E ready-to-use

Type: ELISA

AP0514-050 50 ml
 
Sample & Antibody Diluent E ready-to-use

Type: ELISA

AP0514-125 125 ml
 
Sample & Antibody Diluent E ready-to-use

Type: ELISA

AP0514-500 500 ml
 
Section Block ready-to-use

Type: IHC Buffers

AP0471-050 50 ml
 
Section Block ready-to-use

Type: IHC Buffers

AP0471-125 125 ml
 
Single set for 1 mm puncher with a guide

Type: Tissue Microarrayer Set

TAT06T1 1 set
 
Single set for 1.5mm puncher

Type: Tissue Microarrayer Set

TAT06T1.5 1 set
 
Single set for 2mm puncher

Type: Tissue Microarrayer Set

TAT06T2 1 set
 
Single set for 3mm puncher

Type: Tissue Microarrayer Set

TAT06T3 1 set
 
Single set for 5mm puncher

Type: Tissue Microarrayer Set

TAT06T5 1 set
 
Slide Chamber

Type: Accessories

R21001 Set of 3
 
Stripping Buffer ready-to-use W

Type: Western Blot Buffers

AP0503-050 50 ml
 
Stripping Buffer ready-to-use W

Type: Western Blot Buffers

AP0503-125 125 ml
 
Stripping Buffer ready-to-use W

Type: Western Blot Buffers

AP0503-500 500 ml
 
Universal Buffer

Type: Universal Buffers

AP0530-125 125 ml, 10x
 
Universal Buffer

Type: Universal Buffers

AP0530-500 500 ml, 10x
 
Washing Buffer E PBS-Based 10x

Type: ELISA

AP0515-500 500 ml
 
Washing Buffer W Tris-Based 10x

Type: Western Blot Buffers

AP0502-500 500 ml
 
Western Block, BSA-Free ready-to-use

Type: Western Blot Buffers

AP0500-125 125 ml
 
Western Block, BSA-Free ready-to-use

Type: Western Blot Buffers

AP0500-500 500 ml