Total 839 AA. MW: 94.0 kDa (calculated). UniProtKB acc. No. Q9H1B5 (Gly37-Arg865, lumenal domain, isoform 1, natural variant with Thr305). N-terminal linker (2 extra AA), C-terminal linker (2 extra AA) and C-terminal His-tag (6 extra AA). Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
Purity as determined by densitometric image analysis: >95%
14 % SDS-PAGE separation of Human Xylosyltransferase 2:
1. MW marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and boiled sample, 2.5 μg/lane
3. non-reduced and non-boiled sample, 2.5 μg/lane
< 0.1 EU/ug
Filtered (0.4 μm) and lyophilized in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4
Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Filter your culture media/working solutions containing this product before using in cell culture.
Western blotting, ELISA, Cell culture and/or animal studies
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for 4 days.
Quality Control Test
BCA to determine quantity of the protein.
SDS-PAGE to determine purity of the protein.
LAL TEST to determine endotoxin level.
This product is intended for research use only.