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Manufactured by BioVendor

Xylosyltransferase 2 Human HEK293

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:HEK293
  • Other names:Peptide O-xylosyltransferase 1, Xylosyltransferase II, XT-II, XylT-II, UDP-D-xylose:proteoglycan core protein beta-D-xylosyltransferase
  • Species:Human
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Cat. No. Size Price

RD172125100 0.1 mg
PubMed Product Details
Technical Data


Recombinant protein


Total 839 AA. MW: 94.0 kDa (calculated). UniProtKB acc. No. Q9H1B5 (Gly37-Arg865, lumenal domain, isoform 1, natural variant with Thr305). N-terminal linker (2 extra AA), C-terminal linker (2 extra AA) and C-terminal His-tag (6 extra AA). Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence





˃ 90 % by SDS-PAGE


14 % SDS-PAGE separation of Human Xylosyltransferase 2:
1. MW marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and boiled sample, 2.5 μg/lane
3. non-reduced and non-boiled sample, 2.5 μg/lane


< 1.0 EU/µg


Filtered (0.4 μm) and lyophilized in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4


Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.


Western blotting, ELISA


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the lyophilized protein at -80 °C. Lyophilized protein remains stable until the expiry date when stored at -80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80 °C for long term storage. Reconstituted protein can be stored at 4 °C for three days.

Quality Control Test

BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein. LAL to determine quantity of endotoxin.


This product is intended for research use only.



Xylosyltransferases (XTs) are ubiquitously expressed enzymes that belong to the group of glycosyltransfe­rases. These enzymes initiate the proteoglycans formation by assembly of glycosaminoglycan (GAG) chains to the protein substrate by catalysing the transfer of xylose from the nucleoside diphosphate donor (UDP-xylose) to serine residues in the protein [1, 2]. XTs reside in the endoplasmatic reticulum and/or cis-Golgi, but they are also released into extracellular matrix [3]. The XTs serum activity has been proposed as biomarker of altered proteoglycan metabolism in various diseases, eg. diabetes, systematic sclerosis, liver neoplasia and HTLV-1 retrovirus adhesion [4].

Summary References (3)

References to Xylosyltransferase 2

  • Condac E, Dale GL, Bender-Neal D, Ferencz B, Towner R, Hinsdale ME. Xylosyltransferase II is a significant contributor of circulating xylosyltransferase levels and platelets constitute an important source of xylosyltransferase in serum. Glycobiology. 2009 Aug;19 (8):829-33
  • Gotting C, Kuhn J, Brinkmann T, Kleesiek K. Xylosylation of alternatively spliced isoforms of Alzheimer APP by xylosyltransferase. J Protein Chem. 1998 Apr;17 (3):295-302
  • Ponighaus C, Ambrosius M, Casanova JC, Prante C, Kuhn J, Esko JD, Kleesiek K, Gotting C. Human xylosyltransferase II is involved in the biosynthesis of the uniform tetrasaccharide linkage region in chondroitin sulfate and heparan sulfate proteoglycans. J Biol Chem. 2007 Feb 23;282 (8):5201-6
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