Total 291AA, 33,5 kDa (computed). The AA sequence (14–291) is identical to Swiss-Prot-P25311 (AA18–295 mature Zinc-Alfa-2-Glycoprotein). THIRTEEN extra amino acid were fused with N-terminus (highlighted).
Amino Acid Sequence
Purity as determined by densitometric image analysis: >90%
12% SDS-PAGE separation of Human ZA2G
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 10μg/lane
3. non-reduced and non-heated sample, 10μg/lane
< 0.1 EU/ug
Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5
Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
Western blotting, ELISA, Cell culture and/or animal studies
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.