ApoE3 belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE exists in three major isoforms; E2, E3 and E4, which differ from one another by a single amino-acid substitution. E3 is the most common isoform and is present in 40–90% of the population. Recombinant human ApoE3 is a 34.0 kDa protein containing 299 amino acid residues.