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Distributed product

Blyscan™ sGAG Isolation & Concentration Pack

  • Regulatory status:RUO
  • Type:Colorimetric assay
  • Other names:Glycosaminoglycan, sGAG
  • Species:Multispecies
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RBCB1015 100 samples
PubMed Product Details
Technical Data

Type

Colorimetric assay

Applications

Urine, Synovial fluid, Tissue extract, Cell culture and/or animal studies

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

All components are stable for one year, (from Invoice Date), when stored at 15–25ºC. Do not store below +4ºC. Once opened the glass vial containing sGAG standard should however be stored at +4ºC.

Summary

Features

  • The Blyscan Assay is a quantitative dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans, (sGAG).
  • Test material can be assayed directly when present in a soluble form, or following papain extraction from biological materials. The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples.
  • The dye label used in the assay is 1, 9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains.
  • The assay is not suitable for small sulfated disaccharide fragments or for samples containing alginates, as these contain uronic acid.
  • Time required is 1 hour.
  • Manufactured by Biocolor.

Research topic

Extracellular matrix, Animal studies

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