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BoneTRAP® (TRAcP 5b) ELISA

  • Regulatory status:RUO
  • Type:Immunocapture enzyme activity assay
  • Other names:Tartrate Resistant Acid Phosphatase 5
  • Species:Human
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Cat. No. Size Price


New SB-TR201A 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Immunocapture enzyme activity assay

Applications

Serum, Plasma-EDTA

Sample Requirements

100 µl/well

Shipping

Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

0.5 - 10 U/L

Limit of Detection

< 0.5 U/L

Intra-assay (Within-Run)

n = 21

CV = 9.0%

Inter-assay (Run-to-Run)

n = 11

CV = 7.7%

Spiking Recovery

100.9%

Dilution Linearity

up to 1:5

Summary

Features

European Union: for in vitro diagnostic use

Rest of the world: for research use only!

Derived exclusively from bone-resorbing osteoclasts

Low diurnal variation

No dietary influence

No accumulation into the circulation in renal or hepatic failure

Research topic

Bone and cartilage metabolism

Summary

For the quantitative determination of the active isoform 5b of tartrate-resistant acid phosphatase (TRAcP). The BoneTRAP® (TRAcP 5b) ELISA assay is a specific method to detect TRAcP 5b activity freshly liberated from osteoclasts. It is intended for use as an indicator of bone resorption and can be used as an aid in monitoring bone resorption changes in post-menopausal women and individuals diagnosed with osteoporosis undergoing anti-resorptive therapies. In-vitro, TRAcP 5b activity reflects the number of osteoclasts and therefore the BoneTRAP® (TRAcP 5b) ELISA Assay can be conveniently used to determine osteoclast number in human osteoclast cultures.

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