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Collagen

Soluble and Insoluble Collagen measurement Collagen is the most abundant protein found in animals. During a healthy life span the insoluble, covalent, cross-linked collagen fibers retain their biophysical functions and shapes. The collagen fibers remain isolated from most of the biochemical activities of the cells. Trauma caused by metabolic internal events, external chemical agents or physical injuries however, quickly lead to dramatic activity, resulting in rapid collagen removal followed by a wound healing response (collagen regeneration and associated remodeling). Newly formed soluble collagen production and the residual insoluble collagen fibers can be monitored using the Sircol Soluble Collagen and Sircol Insoluble Collagen Assays. Collagen proteins contain one or more domains with a triple helical structure. The three chains are described as alpha chains and should not be confused with the alpha helixes found in other proteins. The fibrillar collagens (Types I, II, III, V & XI) have most of their alpha chain structure composed of a continuous repeating tri-peptide sequence made up of glycine in every third amino acid residue [(gly-X-Y)n ]. It is to this sequence that the Sircol Dye binds. Proline is frequently an occupant in the ‘Y’ location of the tri-peptide [(gly-X-Y)n]. Many of these residues are converted, post translation, into hydroxyproline residues prior to triple helix formation and the release of the tropocollagen into the ECM. Until now, an investigator seeking to measure covalent cross-linked insoluble collagen required measurements based on free hydroxyproline content – a procedure that requires strong acid (6.0 M HCl), high temperatures (+ 95 °C) and overnight cooking (18 to 24 hrs).

15 results found in Extracellular Matrix Assays

Extracellular Matrix Assays
Product Size Price
S2000 Denatured Collagen Standard (1 mg/ml)

Type: Colorimetric assay

RBCS2010 3x 5 ml
 
S2000 Fragmentation Reagent

Type: Colorimetric assay

RBCSFRAG 3x 110 ml
 
Sircol™ Acid-Salt Wash

Type: Colorimetric assay

RBCSWASH 3x 20 ml
 
Sircol™ Bovine Collagen Reference Standard (0.5 mg/ml)

Type: Colorimetric assay

RBCS1010 3x 5 ml
 
Sircol™ Dye & Dye Release Reagents

Type: Colorimetric assay

RBCS1500 2x 110 ml, 2x 150 ml
 
Sircol™ Dye Reagent

Type: Colorimetric assay

RBCS1005 3x 110 ml
 
Sircol™ Dye Release Reagent

Type: Colorimetric assay

RBCS1007 3x 150 ml
 
Sircol™ Insoluble Collagen Assay (Large Economy Kit)

Type: Colorimetric assay

RBCS6000 400 assays (1 kit)
 
Sircol™ Insoluble Collagen Assay, Bovine Collagen (Standard Kit Size)

Type: Colorimetric assay

RBCS2000 100 assays (1 kit)
 
Sircol™ Isolation & Concentration Reagent

Type: Colorimetric assay

RBCSICR 3x 20 ml
 
Sircol™ Rat Collagen Reference Standard (0.5 mg/ml)

Type: Colorimetric assay

RBCS1011 3x 5 ml
 
Sircol™ Soluble Collagen Assay (Large Economy Kit)

Type: Colorimetric assay

RBCS5000 440 assays (1 kit)
 
Sircol™ Soluble Collagen Assay, Bovine Collagen (Standard Kit Size)

Type: Colorimetric assay

RBCS1000 110 assays (1 kit)
 
Sircol™ Soluble Collagen Assay, Rat Collagen (Standard Kit Size)

Type: Colorimetric assay

RBCS1111 110 assays (1 kit)
 
Sircol™ Soluble plus Sircol™ Insoluble Collagen Combination Pack

Type: Colorimetric assay

RBCS4000 2x 110 assays