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Collagen

Soluble and Insoluble Collagen measurement Collagen is the most abundant protein found in animals. During a healthy life span the insoluble, covalent, cross-linked collagen fibers retain their biophysical functions and shapes. The collagen fibers remain isolated from most of the biochemical activities of the cells. Trauma caused by metabolic internal events, external chemical agents or physical injuries however, quickly lead to dramatic activity, resulting in rapid collagen removal followed by a wound healing response (collagen regeneration and associated remodeling). Newly formed soluble collagen production and the residual insoluble collagen fibers can be monitored using the Sircol Soluble Collagen and Sircol Insoluble Collagen Assays. Collagen proteins contain one or more domains with a triple helical structure. The three chains are described as alpha chains and should not be confused with the alpha helixes found in other proteins. The fibrillar collagens (Types I, II, III, V & XI) have most of their alpha chain structure composed of a continuous repeating tri-peptide sequence made up of glycine in every third amino acid residue [(gly-X-Y)n ]. It is to this sequence that the Sircol Dye binds. Proline is frequently an occupant in the ‘Y’ location of the tri-peptide [(gly-X-Y)n]. Many of these residues are converted, post translation, into hydroxyproline residues prior to triple helix formation and the release of the tropocollagen into the ECM. Until now, an investigator seeking to measure covalent cross-linked insoluble collagen required measurements based on free hydroxyproline content – a procedure that requires strong acid (6.0 M HCl), high temperatures (+ 95 °C) and overnight cooking (18 to 24 hrs).

33 results found in Extracellular Matrix Assays

Extracellular Matrix Assays
Product Size Price
QuickZyme Hydroxyproline Assay Kit

Type: Colorimetric assay

New QZBHYPRO1 96 wells (1 kit)
New QZBHYPRO2 2 x 96 wells (1 kit)
New QZBHYPRO5 5 x 96 wells (1 kit)
 
QuickZyme Sensitive Tissue Collagen Assay Kit

Type: Colorimetric assay

New QZBTISCOL1 96 wells (1 kit)
New QZBTISCOL2 2 x 96 wells (1 kit)
New QZBTISCOL5 5 x 96 wells (1 kit)
 
QuickZyme Sensitive Tissue Hydroxyproline Kit

Type: Colorimetric assay

New QZBTISHYP1 96 wells (1 kit)
New QZBTISHYP2 2 x 96 wells (1 kit)
New QZBTISHYP5 5 x 96 wells (1 kit)
 
QuickZyme Soluble Collagen Assay Kit

Type: Colorimetric assay

New QZBCOL1-1 96 wells (1 kit)
New QZBCOL1-2 2 x 96 wells (1 kit)
New QZBCOL1-5 5 x 96 wells (1 kit)
 
QuickZyme Total Collagen Assay Kit

Type: Colorimetric assay

New QZBTOTCOL1 96 wells (1 kit)
New QZBTOTCOL2 2 x 96 wells (1 kit)
New QZBTOTCOL5 5 x 96 wells (1 kit)
 
QuickZyme Total Protein Assay Kit

Type: Colorimetric assay

New QZBTOTPROT1 96 wells (1 kit)
New QZBTOTPROT2 2 x 96 wells (1 kit)
New QZBTOTPROT5 5 x 96 wells (1 kit)
 
S2000 Denatured Collagen Standard (1 mg/ml)

Type: Colorimetric assay

S2010 3x 5 ml
 
S2000 Fragmentation Reagent

Type: Colorimetric assay

SFRAG 3x 110 ml
 
Sircol Isolation-Concentration & Acid Neutralising Reagents (2x2x20 ml)

Type: Colorimetric assay

New SCONC 2x2x20 ml
 
Sircol™ Acid-Salt Wash

Type: Colorimetric assay

SWASH 3x 20 ml
 
Sircol™ Bovine Collagen Reference Standard (0.5 mg/ml)

Type: Colorimetric assay

S1010 3x 5 ml
 
Sircol™ Dye & Dye Release Reagents

Type: Colorimetric assay

S1500 2x 110 ml, 2x 150 ml
 
Sircol™ Dye Reagent

Type: Colorimetric assay

S1005 3x 110 ml
 
Sircol™ Dye Release Reagent

Type: Colorimetric assay

S1007 3x 150 ml
 
Sircol™ Insoluble Collagen Assay (Large Economy Kit)

Type: Colorimetric assay

S6000 400 assays (1 kit)
 
Sircol™ Insoluble Collagen Assay, Bovine Collagen (Standard Kit Size)

Type: Colorimetric assay

S2000 100 assays (1 kit)
 
Sircol™ Rat Collagen Reference Standard (0.5 mg/ml)

Type: Colorimetric assay

S1011 3x 5 ml
 
Sircol™ Soluble Collagen Assay (Large Economy Kit)

Type: Colorimetric assay

S5000 440 assays (1 kit)
 
Sircol™ Soluble Collagen Assay, Bovine Collagen (Standard Kit Size)

Type: Colorimetric assay

S1000 110 assays (1 kit)
 
Sircol™ Soluble Collagen Assay, Rat Collagen (Standard Kit Size)

Type: Colorimetric assay

S1111 110 assays (1 kit)
 
Sircol™ Soluble plus Sircol™ Insoluble Collagen Combination Pack

Type: Colorimetric assay

S4000 2x 110 assays