Type
Sandwich ELISA, HRP-labelled antibody
Description
This ELISA kit is used for quantitative determination of glucagon in plasma, serum and culture medium supernatant. The kit is characterized by its sensitive quantification and high specificity. In addition, it has no influence by other components in samples. Glucagon standard is highly purified synthetic product.
Applications
Serum, Plasma, Cell culture supernatant
Sample Requirements
10 ul
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at 2–8°C. Under these conditions, the kit is stable until expiration date (see label on the box).
Calibration Range
2.2 -143.6 pmol/L (7.8 - 500 pg/mL)
Limit of Detection
0.3 pmol/L (1.08 pg/mL)
Intra-assay (Within-Run)
serum CV = 1.8-3.5%, plasma CV = 2.1- 4.6%
Inter-assay (Run-to-Run)
serum CV = 3.5-9.1%, plasma CV = 3.4-7.1%
Specificity
This ELISA kit has high specificity to glucagon, and shows no significant cross reactivity to Glicentin, Oxyntomodulin, GLP-1 and GLP-2.
Features
- RUO
- one step sandwich ELISA
- assay kit can measure Glucagon within the range of 2.2 -143.6 pmol/L
- sensitivity : 0.3 pmol/L
- the assay is completed within 18-20hr + 0.5hr.
- with one assay kit, 40 samples can be measured in duplicate
Research topic
Diabetology - Other Relevant Products, Animal studies
Summary
Glucagon is a 29-amino acid polypeptide hormone, synthesized and secreted from alpha cells of the
islets of Langerhans. Glucagon generally elevates the concentration of glucose in the blood by
promoting gluconeogenesis and glycogenolysis, and it has the opposite effect of insulin. The secretion of glucagon and insulin into the blood in response to the blood glucose concentration is the primary
mechanism responsible for keeping the glucose levels.
This Glucagon sandwich ELISA kit has been developed by using two monoclonal antibodies specific for
glucagon. This kit has high specificity to glucagon and shows no significant cross reactivity with
Glicentin, Oxyntomodulin, GLP-1 and GLP-2.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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