Type
Recombinant protein
Description
Fibronectin Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 574 amino acids and having a molecular mass of 62.6kDa. The Fibronectin is purified by proprietary chromatographic techniques.
Fibronectin is useful for the induction of cell attachment to a variety of surfaces including plastic and glass tissue culture labware, petri dishes, coverslips, microcarrier beads, etc. Fibronectin is useful for growth and maintenance of cells in low serum conditions. In general, the reconstituted fibronectin should be diluted with sterile physiological saline or serum-free medium to a concentration of 10-50ug/ml.
Source
E. coli
Purity
Greater than 95.0%
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 5 % Trehalose and 0.02 % Tween-20.
Reconstitution
It is recommended to reconstitute the lyophilized Fribronectin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Applications
Cell culture and/or animal studies, In vitro
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized Fibronectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibronectin should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Physical Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Note
This BioVendor product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.