Type
Sandwich ELISA, AChE-labelled antibody
Applications
Plasma-EDTA
Sample Requirements
10 µl/well
Shipping
On ice. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at -20°C. Under these conditions, the kit is stable until expiration date (see label on the box).
Calibration Range
1.96–250 pg/ml
Limit of Detection
The Limit of Determination, calculated as the concentration of unacylated ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 0.6 pg/ml and 0.8 pg/ml for long and short first incubation period, respectively.
Intra-assay (Within-Run)
CV = 8.2% (20h at +4°C)
CV = 9.2% (3h at RT)
Inter-assay (Run-to-Run)
CV = 8.6% (20h at +4°C)
CV = 9.5% (3h at RT)
Spiking Recovery
101.5% (20h at +4°C)
99.8% (3h at RT)
Dilution Linearity
98,90%