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Ghrelin Unacylated Mouse/Rat Express ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, AChE-labelled antibody
  • Other names:M46, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46, GHRL, MTLRP, UNQ524/PRO1066
  • Species:Rat
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Cat. No. Size Price


RA394063400R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, AChE-labelled antibody

Applications

Plasma-EDTA

Sample Requirements

10 µl/well

Storage/Expiration

Store the kit at –20°C. Under these conditions, the kit is stable until expiration date (see label on the box).

Calibration Range

1.96–250 pg/ml

Limit of Detection

The Limit of Determination, calculated as the concentration of unacylated ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 0.6 pg/ml and 0.8 pg/ml for long and short first incubation period, respectively.

Intra-assay (Within-Run)

CV = 8.2% (20h at +4°C)
CV = 9.2% (3h at RT)

Inter-assay (Run-to-Run)

CV = 8.6% (20h at +4°C)
CV = 9.5% (3h at RT)

Spiking Recovery

101.5% (20h at +4°C)
99.8% (3h at RT)

Dilutation Linearity

98,90%

Summary

Features

  • The kit measures plasma unacylated ghrelin.
  • Quality controls are rat-plasma based.

Research topic

Animal studies, Diabetology - Other Relevant Products, Energy metabolism and body weight regulation

Summary

Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that controls energy homeostasis. The peptide consists of 28 amino acids, with a n-octanoylation of the serine-3 residue, which is necessary for the biological activity mentioned below. Ghrelin is present in the peripheral circulation under two forms: acylated and unacylated.

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