Type
Sandwich ELISA, Biotin-labelled antibody
Description
The assay is based on polyclonal antibodies and recombinant IGFBP-6,
expressed in eukaryotic cells. The ELISA for IGFBP-6, E112 is a so-called
Sandwich-Assay.
Applications
Serum, Plasma, Body fluids
Sample Requirements
10 μl
Shipping
Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Range
0.1 – 10 ng/ml
Limit of Detection
0.026 ng/ml
Intra-assay (Within-Run)
<10 %
Inter-assay (Run-to-Run)
<10 %
Features
For Research Use Only
incubation period 3 hours
buffer : RTU and 20fold concentrate
2 control sera, freeze-dried
Intra- / Interassay Variance <10 %
Research topic
Animal studies
Summary
The Insulin-like Growth Factor 6 (IGFBP-6) is part of the IGF-System which consists of
several binding proteins (1-6) and IGF-I and –II. The Insulin-like Growth Factors are involved
in the control of human growth and regulated themselves by their binding proteins. The
IGFBPs show high amino acid sequence homology, they have conserved N- and C-terminal
domains which are involved in IGF-binding. In contrast the central linker region is highly
variable. This region is not directly involved in IGF-binding but influences stability and
localisation of the IGFBP / IGF complex and can be modified by glycosylation and
phosphorylation.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
MSDS (RUO)
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