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Manufactured by BioVendor

Leptin Receptor Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Species:Human
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Cat. No. Size Price


RD194002100 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin

Sample Requirements

35 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

2–100 ng/ml

Limit of Detection

Analytical Limit of Detection is calculated from the real Leptin receptor values in wells and is 0.04 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 7.2%

Inter-assay (Run-to-Run)

n = 7; CV = 8.0%

Spiking Recovery

88,60%

Dilutation Linearity

107,90%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • horse Non-detectable
  • hamster Not tested
  • monkey Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • human Yes
Summary

Features

  • For research use only !
  • The total assay time is less than four hours.
  • The kit measures total serum Leptin receptor.
  • Standard is recombinat protein based.
  • Quality Controls are human serum based.
  • Components of the kit are ready-to-use (with the exception of Wash Solution and Quality Controls).

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Reproduction

Summary

Leptin receptor (OB R) was identified as a leptin binding protein (Leptin, the product of the ob gene, is a single chain 16 kDa protein consisting of 146 amino acid residues.) OB R was found to be a member of the class I cytokine receptor family with a large extracellular domain comprising 816 amino acid residues. Leptin receptor exists in multiple forms with a common extracellular domain and a variable length cytoplasmatic portion. Alternate splicing from a single gene derives the six isoforms of the Leptin receptor. The soluble form of the Leptin receptor, OB R contains no intracellular motifs or transmembrane residues, thus it consists entirely of the extracellular ligand binding domain of the receptor. Long forms of OB-R transcripts were reported to be expressed predominantly in regions of the hypothalamus which provides evidence that Leptin receptor is important in body weight regulation. Expression of short forms of OB-R transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. Leptin receptor may act as a negative regulator of Leptin activity and it may maintain a pool of available bioactive Leptin by binding and delaying its clearance from circulation. Soluble Leptin receptor levels are indirectly proportional to adiposity and are increased in females versus males. Leptin receptor levels are highest in infants, decrease into adolescence, and remain relatively stable throughout adulthood. Soluble Leptin receptor is also found upregulated in patients with chronic heart failure, end-stage renal disease and anorexia.

References to Product

References

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References to Summary

References to Leptin Receptor

  • Lahlou N. et al.: Soluble leptin receptor in serum of subjects with complete resistance to leptin: Diabetes; 19:1347–1352 (2000)
  • Auwerx J. and Staels B.: Leptin (Review article). The Lancet; 13:737 (1998)
  • Chen H. et al.: Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice. Cell; 84:491–495 (1996)
  • Ciofii J. A. et al.: Novel B219/OB receptor isoforms: possible role of leptin in hematopoiesis and reproduction. Nature Med.; 2:585–589 (1996)
  • Houseknecht K. L. and Portocarrero C. P.: Leptin and its receptors: Regulators of whole body energy homeostasis. Domestic animal endocrinology; 15 (6):457–475 (1998)
  • Lee G.H. et al.: Abnormal splicing of the leptin receptor in diabetic mice. Nature; 379:632–635 (1996)
  • Tartaglia L. A. et al.: Identification and Expresion Cloning of a Leptin Receptor, OB-R. Cell; 83:1263–1271 (1995)
  • Tartaglia L. A.: The leptin receptor. J. Biol. Chem.; 272:6093–6096 (1997)
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