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sFas Ligand Human ELISA

Other names: CD178 antigen, CD95L protein Product of BioVendor
Product: Size:
RAF100R (regulatory status: RUO) 96 wells (1 kit)
Files: Datasheet PDF (RUO)MSDS (RUO) Fas Ligand on pubmed

Product details


Summary

Fas is a type I membrane protein that belongs to the TNF/nerve growth factor receptor family. Fas mediates apoptosis, the programmed cell death, when it is cross-linked with specific binding partners. FasL is predominantly expressed on activated T-cells and NK cells; thus FasL-mediated cell death is involved in the T or NK cell-mediated cytotoxicity, some pathologic tissue damages, and the regulation of lymphocyte homeostasis. Elevated expression levels of FasL have been measured in various proliferative disorders and cancers.

Research topic

Apoptosis


Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Amniotic fluid, Cell culture supernatant, Plasma, Serum

Sample requirements

50 µl/well

Storage/Shipping

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration range

0.16–10.0 ng/mL

Limit of detection

0.07 ng/ml

Intra-assay

CV = 6.1%

Inter-assay

CV = 7.0%

Spiking Recovery

85.0%

Dilution Linearity

106.0%


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By molecule:


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