Recombinant Factor C Endotoxin Detection Kit

Features

• It is intended for research use only.
• Recombinant Factor C Endotoxin Detection Kit is intended for in vitro quantitative determination of lipopolysaccharide (LPS) in aqueous samples and biological fluids in non-regulated fields.
• Recombinant Factor C Endotoxin Detection Kit is not intended for the diagnosis of human or animal diseases and is not suitable for the detection of endotoxin in serum, plasma and blood samples.
• Two-point end-point fluorescence assay
• High sensitive assay
• No washing step
• Robust assay with excellent reproducibility, high stability of endotoxin standard after reconstitution
• Broad measurement range (0.005–5 EU/ml)
• pH range (pH6 – pH8), excitation/emission wavelength of 380/440nm
• All reagents necessary to run the assay included in the kit
• Uses recombinant Factor C (rFC), thus saving the diminishing horseshoe crab population

Summary

The BioVendor Recombinant C Factor (rFC) Endotoxin Detection Kit is based on a fluorescence enzymatic reaction using recombinant Factor C.

Assay principle:

The kit contains recombinant Factor C, a genetically engineered protein that specifically recognizes endotoxins (lipopolysaccharides, LPS) from Gram-negative bacteria. If endotoxin is present in the sample, it binds to and activates recombinant Factor C. The activated Factor C cleaves a fluorogenic substrate included in the kitresulting in a fluorescent signal. The fluorescence intensity is directly proportional to the endotoxin concentration and is quantified using a calibration curve.