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References

  • Buchacher T, Wiesinger-Mayr H, Vierlinger K, Ruger BM, Stanek G, Fischer MB, Weber V. Human blood monocytes support persistence, but not replication of the intracellular pathogen C. pneumoniae. BMC Immunol. 2014;15:60
  • Charwat V, Schutze K, Holnthoner W, Lavrentieva A, Gangnus R, Hofbauer P, Hoffmann C, Angres B, Kasper C. Potential and limitations of microscopy and Raman spectroscopy for live-cell analysis of 3D cell cultures. J Biotechnol. 2015 Jul 10;205:70–81
  • Smith R, Wright KL, Ashton L. Raman spectroscopy: an evolving technique for live cell studies. Analyst. 2016 Jun 21;141(12):3590-600
  • Steinke M, Gross R, Walles H, Gangnus R, Schutze K, Walles T. An engineered 3D human airway mucosa model based on an SIS scaffold. Biomaterials. 2014 Aug;35 (26):7355–62
microRNA IN A NEW LIGHT!

microRNA IN A NEW LIGHT!

MicroRNAs (miRNAs) are new and promising potential biomarkers for diagnosis and prognosis of many diseases, which opens up a new field of goals and challenges for the BioVendor R&D team. Development of miRNA diagnostic assays is just the first step; our mission is to provide validated kits to measure individual miRNAs or diagnostically relevant miRNA “groups” supported with tools for standardized miRNA isolation, sample collection, and pretreatment.

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MicroRNAs (miRNAs) are small, non-coding RNA molecules typically containing ~22 nucleotides found in human, animals, plants and some viruses which are playing an important regulatory role in the gene translation via silencing or degradation of target mRNAs. During the RNA silencing, miRNA functions as a complementary guide which binds with its target mRNAs, followed by action of AGO proteins family which works as effectors by recruiting factors that induce translational repression, mRNA deadenylation and mRNA decay. (Minju Ha, Nature Reviews, 2014)

MicroRNAs (miRNAs) are involved in virtually all physiologic processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation. The biogenesis and function of miRNAs are tightly regulated, and their dysregulation is often associated with pathology of many diseases such as cancer, heart disease or neurological disorders. Recently, the miRNA has been proposed as powerful biomarker for prediction of the diseases, treatment response or prediction of progression.

 

ID3EAL Biofluid miRNA Panel

ID3EAL Biofluid miRNA Panel

MiRXES ID3EAL Biofluid miRNA knowledge panel was compared to equivalent products from two leading vendors using a common pool of human serum RNA. For each product, RNA extracted from 200 μL of pooled human serum was used at the start of respective workflows. Detection cut-offs were determined based on the respective manufacturers’ instructions (CT ≤ 33 for ID3EAL Biofluid Panel). Of the 3 biofluid miRNA products, the ID3EAL Biofluid Panel detected the highest number of microRNA.

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Separately, the ID3EAL Biofluid Panel was used to illustrate distinct miRNA profiles in normal and gastric cancer patient serum.
11030
Format of Panel

 

11051 

Biofluid miRNA knowledge panel

 

CAT. NO. Name DESCRIPTION SAMPLES
1105151 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (2) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
2 x 96-well PCR plates
2
1105152 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
6 x 96-well PCR plates
6
1105153 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
12
1105154 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
24
1105251 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (2) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
4 x 96-well PCR plates
2
1105252 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (6) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
6
1105253 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (12) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
12
1105254 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (24) 4 x 24 μl of ID3EAL miRNA RT Primers 96-plex
48 x 96-well PCR plates
24

 

Choose your thermal cycler

  • Roche
  • Abi Fast
  • Abi Standard
  • Bio-Rad

Biofluid miRNA Panel Analysis Template 

 

ID3EAL Cancer miRNA Panel

ID3EAL Cancer miRNA Panel

Four distinct cell types (HDFa, BE2C, HCT116 and U251) were profiled with the ID3EAL Cancer miRNA Panel. One hundred nanograms of RNA from each sample was used for reverse transcription. About 300 miRNAs were detected for each cell line, with 244 common miRNAs detected with CT ≤ 33 across all 4 cell lines.

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Number of miRNAs Detected                                                  CT number of miRNAs Detected

Inter-plate calibrator normalization and biological normalization by global mean or reference miRNAs were performed prior to inter-sample miRNA ΔCT calculation. MiRNA expression comparison in HFDa and U251 cells are visualized through a scatter plot. Data points that fall outside of ±1 cycles regression lines indicate miRNAs differentially expressed in the both cell lines.


11049

Format of Panel

11051

 Cancer miRNA knowledge panel 

CAT. NO. Name DESCRIPTION SAMPLES
1105151 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (2) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
2 x 96-well PCR plates
2
1105152 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
6 x 96-well PCR plates
6
1105153 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
12
1105154 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
24
1105261 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (2) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
8 x 96-well PCR plates
2
1105262 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (6) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
6
1105263 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (12) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
48 x 96-well PCR plates
12
1105264 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
96 x 96-well PCR plates
24


Choose your thermal cycler

  • Roche
  • Abi Fast
  • Abi Standard
  • Bio-Rad

Cancer miRNA Panel Analysis Template