Type
Recombinant protein
Description
Recombinant Human SCF produced in E.Coli is a single, non-glycosylated polypeptide chain containing 164 amino acids and having a molecular mass of 18.4 kDa. The SCF is purified by proprietary chromatographic techniques.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Gly-Ile-Cys.
Source
E. coli
Purity
Greater than 98.0% as determined by: - (a) Analysis by RP-HPLC. - (b) Anion-exchange FPLC. - © Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Biological Activity
BioVendor´s recombinant human SCF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 5×105 IU/mg.
Endotoxin
Less than 0.1 ng/Cg (IEU/Cg) of rHuSCF.
Formulation
Lyophilized from a concentrated (1mg/ml) solution in water containing 10mM Acetic acid.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized rHuSCF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuSCF should be stored at 4°C between 2–7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance
Sterile filtered white lyophilized (freeze-dried) powder.