Biotinylated recombinant human Angiotensin-converting enzyme 2 (ACE2) expressed in HEK293 cells. Extracellular domain of human ACE2 (aa 18-740) are fused at the C-terminus to a FLAG®-tag. Molecular weight: ~110kDa (SDS-PAGE); ~240kDa (Dimer, by SEC).
ACE2 Human, HEK293 Recombinant binds with high affinity to the Spike S1RBD protein of the virus SARS-CoV-2.
ACE2 Human, HEK293 Recombinant was coated on an ELISA plate at 1μg/ml overnight at 4°C. Recombinant SARS-CoV-2 Spike Protein S1RBD was added (starting at a concentration of 500ng/ml with a two-fold serial dilution) during one hour at RT and the interaction was then detected using an anti-human IgG (HRP).
<0.01 EU/μg (LAL test).
1 mg/ml after reconstitution. Lyophilized. Contains PBS.
Reconstitute with 50 μl endotoxin-free water.
Cell culture and/or animal studies, In vitro, COVID-19
Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.
Stable for at least 6 months after receipt when stored at -20°C. After opening, prepare aliquots and store at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.
This BioVendor product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.