Type
Recombinant protein
Description
Total 226 AA, UniProt Q15848 (Glu19 – Asn244). MW (monomer): 24.6 kDa (calculated). It migrates at 884 kDa (multimer).
Amino Acid Sequence
ETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN
Glu 1 to Gln 5 were confirmed by N-terminal sequencing
Source
HEK293
Purity
˃ 90 % by SDS-PAGE
SDS-PAGE Gel
4–20% gradient SDS-PAGE separation Adiponectin HMW-rich Human HEK293, Tag free
1. M.W. marker –14, 21, 31, 45, 66, 97 kDa
2. reduced and boiled sample, 2.5μg/lane
3. non-reduced and non-boiled sample, 2.5μg/lane
Biological Activity
Effect of this product on PDGF-induced proliferation of human aortic smooth muscle cells was confirmed experimentaly.
Endotoxin
< 1.0 EU/µg
Formulation
Filtered (0.4 μm) and lyophilized in 20 mM Tris, 50 mM NaCl, pH 7.5 and 1mM CaCl2 at 0.5 mg/mL concentration.
Reconstitution
Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.
Applications
Western blotting, ELISA, Cell culture and/or animal studies
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
GFC to determine purity of the protein.
LAL to determine quantity of endotoxin.