Total 226 AA, UniProt Q15848 (Glu19 – Asn244). MW (monomer): 24.6 kDa (calculated). It migrates at 884 kDa (multimer).
Amino Acid Sequence
Glu 1 to Gln 5 were confirmed by N-terminal sequencing
˃ 90 % by SDS-PAGE
4–20% gradient SDS-PAGE separation Adiponectin HMW-rich Human HEK293, Tag free
1. M.W. marker –14, 21, 31, 45, 66, 97 kDa
2. reduced and boiled sample, 2.5μg/lane
3. non-reduced and non-boiled sample, 2.5μg/lane
Effect of this product on PDGF-induced proliferation of human aortic smooth muscle cells was confirmed experimentaly.
< 1.0 EU/µg
Filtered (0.4 μm) and lyophilized in 20 mM Tris, 50 mM NaCl, pH 7.5 and 1mM CaCl2 at 0.5 mg/mL concentration.
Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.
Western blotting, ELISA, Cell culture and/or animal studies
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein. Endotoxin level determination.