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Manufactured by BioVendor

Alpha-Fetoprotein Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:AFP
  • Species:Human
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Cat. No. Size Price


RIS0018R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum

Sample Requirements

25 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

10–160 IU/ml

Limit of Detection

1 IU/ml

Summary

Research topic

Oncology

Summary

Alpha-fetoprotein (AFP) is a glycoprotein with a molecular weight of approximately 70 KD. AFP is normally produced during fetal and neonatal development by the liver, yolk sac, and in small concentrations by the gastrointestinal tract. After birth, serum AFP concentrations decrease rapidly, and by the second year of life and thereafter only trace amounts are normally detected in serum. Elevation of serum AFP to abnormally high values occurs in several malignant diseases, most notably nonseminomatous testicular cancer and primary hepatocelluar carcinoma. In the case of nonseminomatous testicular cancer, a direct relationship has been observed between the incidence of elevated AFP levels and the stage of disease. Elevated AFP levels have also been observed in patients diagnosed with seminoma with nonseminomatous elements, but not in patients with pure seminoma. In addition, elevated serum AFP concentrations have been measured in patients with other noncancerous diseases, including ataxia telangiectasia, hereditary tyrosinemia, neonatal hyperbilirubinemia, acute viral hepatitis, chronic active hepatitis and cirrhosis. Elevated serum AFP concentrations are also observed in pregnant women. Therefore, AFP measurements are not recommended for use as a screening procedure to detect the presence of cancer in the general population.

References to Summary

References to Alpha-Fetoprotein

  • Abelev GI. Alpha-fetoprotein in ontogenesis and its association with malignant tumors. Adv Cancer Res. 1971;14:295-358
  • Belanger L. Tyrosinemie Hereditaire et Alpha-1-Fetoproteine. II. Recherche Tissulaire Comparee de I'Alpha-Foetoproteine dans Deux Cas de Tyrosineimie Hereditaire. Considerations sur I'Ontogenese de la Foetoproteine Humain (French). Pathol. Bio. 1973;21:457
  • Bosl GJ, Lange PH, Fraley EE, Goldman A, Nochomovitz LE, Rosai J, Waldmann TA, Johnson K, Kennedy BJ. Human chorionic gonadotropin and alphafetoprotein in the staging of nonseminomatous testicular cancer. Cancer. 1981 Jan 15;47 (2):328-32
  • Endo Y, Kanai K, Oda T, Mitamura K, Iino S. Clinical significance of alpha-fetoprotein in hepatitis and liver cirrhosis. Ann N Y Acad Sci. 1975 Aug 22;259:234-8
  • Engvall E. Enzyme immunoassay ELISA and EMIT. Methods Enzymol. 1980;70 (A):419-39
  • Fukuda H, Yano M, Hyouchi N, Noro A, Suzuki S. [A case of nonseminomatous germ cell tumor: usefulness of alphafetoprotein subfraction]. Hinyokika Kiyo. 1994 Apr;40 (4):357-60
  • Gitlin D, Perricelli A, Gitlin GM. Synthesis of -fetoprotein by liver, yolk sac, and gastrointestinal tract of the human conceptus. Cancer Res. 1972 May;32 (5):979-82
  • Javadpour N, McIntire KR, Waldmann TA. Human chorionic gonadotropin (HCG) and alpha-fetoprotein (AFP) in sera and tumor cells of patients with testicular seminoma: a prospective study. Cancer. 1978 Dec;42 (6):2768-72
  • Kew MC, Purves LR, Bersohn I. Serum alpha-fetoprotein levels in acute viral hepatitis. Gut. 1973 Dec;14 (12):939-42
  • Kohn J, Orr AH, McElwain TJ, Bentall M, Peckham MJ. Serum-alpha1-fetoprotein in patients with testicular tumours. Lancet. 1976 Aug 28;2 (7983):433-6
  • Masseyeff R, Gilli G, Krebs B, Bonet C, Zrihen H. Evolution en Fonction de I'Age du Taux Serique Physiologique de I'Alpha-Foetoproteine chez I'Homme et le Rat. (French) in Alpha-Feto-Protein. INSERM. 1974;:313
  • Purves LR, Purves M. Serum alpha-feto-protein. VI. The radio-immunoassay evidence for the presence of AFP in the serum of normal people and during pregnancy. S Afr Med J. 1972 Sep 9;46 (36):1290-7
  • Ruoslahti E, Seppala M. Studies of carcino-fetal proteins: physical and chemical properties of human alpha-fetoprotein. Int J Cancer. 1971 Mar 15;7 (2):218-25
  • Scardino PT, Cox HD, Waldmann TA, Mcintire KR, Mittemeyer B, Javadpour N. The value of serum tumor markers in the staging and prognosis of germ cell tumors of the testis. J Urol. 1977 Dec;118 (6):994-9
  • Seppala M, Ruoslahti E. Alpha fetoprotein: Physiology and pathology during pregnancy and application to antenatal diagnosis. J Perinat Med. 1973;1 (2):104-13
  • Silver HK, Gold P, Feder S, Freedman SO, Shuster J. Radioimmunoassay for human alpha 1 -fetoprotein. Proc Natl Acad Sci U S A. 1973 Feb;70 (2):526-30
  • Uotila M, Ruoslahti E, Engvall E. Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. J Immunol Methods. 1981;42 (1):11-5
  • Waldmann TA, McIntire KR. The use of a radioimmunoassay for alpha-fetoprotein in the diagnosis of malignancy. Cancer. 1974 Oct;34 (4 Suppl):suppl:1510-5
  • Waldmann TA, McIntire KR. Serum Alpha-Fetoprotein Levels in Patients with Ataxia-Telangiectasia. Lancet. 2:1112, 1972
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