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Anti-OVA-IgE Mouse ELISA

  • Regulatory status:RUO
  • Type:Direct ELISA, Biotin-labelled antibody
  • Other names:anti-Ovalbumin
  • Species:Mouse
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RSHAKRIE030R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Direct ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

5 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1.88–120 U/ml

Limit of Detection

0.094 U/ml

Intra-assay (Within-Run)

n = 5; CV = 5%

Inter-assay (Run-to-Run)

n = 3; CV = 5%

Summary

Research topic

Animal studies

Summary

IgE (immunoglobulin E) is the 5th immunoglobulin found and is composed of 2 heavy chains which contain 5 domains (VH, CH 1-4) and 2 light chains. It is a glycoprotein with molecular weight of 190kDa, and in electrophoresis it moves to 1 region. Metabolic half life of IgE is about 3 days in man, and normal serum level in human is about 300 ng/ml, however, it is markedly increased in parasite infection and hay fever. Allergy-related IgE is called reagin. The Fc region of reagin increased after sensitization with allergens will bind Fc R1 receptor of basophilic granulocytes and mast cells in the skin, respiratory, and digestive organs, and sensitizes those cells.
Those IgE-sensitized cells will be degranulated when the second allergens bind the surface IgE, and release histamine, serotonine, protease, heparin, chemotactic factors, prostaglandins, leucotriens, etc., causing type I allergy reactions. Shibayagi’s OVA-IgE ELISA KIT is a useful tool for studying mouse immune system by measuring specific anti-OVA IgE after immunization of mice with OVA (ovalbumin).

References to Product

References

  • Akiyama K, Karaki M, Kobayshi R, Dobashi H, Ishida T, Mori N. IL-16 variability and modulation by antiallergic drugs in a murine experimental allergic rhinitis model. Int Arch Allergy Immunol. 2009;149 (4):315-22
  • Fukui N, Honda K, Ito E, Ishikawa K. Peroxisome proliferator-activated receptor gamma negatively regulates allergic rhinitis in mice. Allergol Int. 2009 Jun;58 (2):247-53
  • He M, Ichinose T, Yoshida S, Nishikawa M, Mori I, Yanagisawa R, Takano H, Inoue K, Sun G, Shibamoto T. Urban particulate matter in Beijing, China, enhances allergen-induced murine lung eosinophilia. Inhal Toxicol. 2010 Aug;22 (9):709-18
  • Ichinose T, Yoshida S, Hiyoshi K, Sadakane K, Takano H, Nishikawa M, Mori I, Yanagisawa R, Kawazato H, Yasuda A, Shibamoto T. The effects of microbial materials adhered to Asian sand dust on allergic lung inflammation. Arch Environ Contam Toxicol. 2008 Oct;55 (3):348-57
  • Ichinose T, Yoshida S, Sadakane K, Takano H, Yanagisawa R, Inoue K, Nishikawa M, Mori I, Kawazato H, Yasuda A, Shibamoto T. Effects of asian sand dust, Arizona sand dust, amorphous silica and aluminum oxide on allergic inflammation in the murine lung. Inhal Toxicol. 2008 May;20 (7):685-94
  • Matsumoto K, Inoue H, Fukuyama S, Kan-O K, Eguchi-Tsuda M, Matsumoto T, Moriwaki A, Nakano T, Nakanishi Y. Frequency of Foxp3+CD4CD25+ T cells is associated with the phenotypes of allergic asthma. Respirology. 2009 Mar;14 (2):187-94
  • Saito K, Torii M, Ma N, Tsuchiya T, Wang L, Hori T, Nagakubo D, Nitta N, Kanegasaki S, Hieshima K, Yoshie O, Gabazza EC, Katayama N, Shiku H, Kuribayashi K, Kato T. Differential regulatory function of resting and preactivated allergen-specific CD4+ CD25+ regulatory T cells in Th2-type airway inflammation. J Immunol. 2008 Nov 15;181 (10):6889-97
  • Saito K, Torii M, Ma N, Tsuchiya T, Wang L, Hori T, Nagakubo D, Nitta N, Kanegasaki S, Hieshima K, Yoshie O, Gabazza EC, Katayama N, Shiku H, Kuribayashi K, Kato T. Differential regulatory function of resting and preactivated allergen-specific CD4+ CD25+ regulatory T cells in Th2-type airway inflammation. J Immunol. 2008 Nov 15;181 (10):6889-97
  • Yanase Y, Hiragun T, Uchida K, Ishii K, Oomizu S, Suzuki H, Mihara S, Iwamoto K, Matsuo H, Onishi N, Kameyoshi Y, Hide M. Peritoneal injection of fucoidan suppresses the increase of plasma IgE induced by OVA-sensitization. Biochem Biophys Res Commun. 2009 Sep 25;387 (3):435-9
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